The functional interplay of low molecular weight thiols in Mycobacterium tuberculosis

Journal of Biomedical Science
Carine Sao EmaniBienyameen Baker

Abstract

Three low molecular weight thiols are synthesized by Mycobacterium tuberculosis (M.tb), namely ergothioneine (ERG), mycothiol (MSH) and gamma-glutamylcysteine (GGC). They are able to counteract reactive oxygen species (ROS) and/or reactive nitrogen species (RNS). In addition, the production of ERG is elevated in the MSH-deficient M.tb mutant, while the production of MSH is elevated in the ERG-deficient mutants. Furthermore, the production of GGC is elevated in the MSH-deficient mutant and the ERG-deficient mutants. The propensity of one thiol to be elevated in the absence of the other prompted further investigations into their interplay in M.tb. To achieve that, we generated two M.tb mutants that are unable to produce ERG nor MSH but are able to produce a moderate (ΔegtD-mshA) or significantly high (ΔegtB-mshA) amount of GGC relative to the wild-type strain. In addition, we generated an M.tb mutant that is unable to produce GGC nor MSH but is able to produce a significantly low level of ERG (ΔegtA-mshA) relative to the wild-type strain. The susceptibilities of these mutants to various in vitro and ex vivo stress conditions were investigated and compared. The ΔegtA-mshA mutant was the most susceptible to cellular stress relative...Continue Reading

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Citations

Mar 12, 2021·Metallomics : Integrated Biometal Science·James P C CoverdalePeter J Sadler
Apr 8, 2021·Microbiology·Leah Isobella Rankine-WilsonYossef Av-Gay
Jun 6, 2021·Redox Biology·Joanne Wei Kay Ku, Yunn-Hwen Gan

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