The functional organization of the vestigial locus in Drosophila melanogaster

Molecular & General Genetics : MGG
J A WilliamsJ B Bell

Abstract

Vestigial mutants are associated with imaginal disc cell death which results in the deletion of adult wing and haltere structures. The vestigial locus has previously been cloned, and mutational lesions associated with a number of vg alleles were mapped within a 19 kb DNA region defined as essential for vg function. Herein we report the identification and characterization of a developmentally regulated 3.8 kb vg transcript which is spliced from exons distributed throughout the essential interval defined above. All the characterized classical alleles have predictable effects on this transcription unit, and the severity of this effect is directly proportional to the severity of the wing phenotype. A repetitive domain within this transcription unit was identified and may serve as a tag to isolate other genes with functions related to vg. We also report an exceptional vg allele (vg83b27) that produces an extreme wing and haltere phenotype, but which defines a second vg complementation unit. This allele is associated with a 4 kb deletion entirely within a 4.5 kb vg intron as defined by the 3.8 kb transcription unit. Molecular and genetic evidence indicates that the vg83b27 mutation has a functional 3.8 kb transcription unit, thus acc...Continue Reading

References

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Citations

Apr 24, 1996·Molecular & General Genetics : MGG·A ZiderJ Silber
Nov 18, 2006·Genome Génome / Conseil National De Recherches Canada·Kyle J AndersonRoss B Hodgetts
Feb 5, 2000·Genome Génome / Conseil National De Recherches Canada·V K LloydT A Grigliatti
Jun 10, 2005·Genetics·Alistair B CoulthardArthur J Hilliker
Dec 13, 2001·Developmental Cell·V SudarsanH Skaer
Apr 27, 2016·Development Genes and Evolution·Emilie SimonPierre Thiébaud

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