The functional structure of central carbon metabolism in Pseudomonas putida KT2440

Applied and Environmental Microbiology
Suresh SudarsanAndreas Schmid

Abstract

What defines central carbon metabolism? The classic textbook scheme of central metabolism includes the Embden-Meyerhof-Parnas (EMP) pathway of glycolysis, the pentose phosphate pathway, and the citric acid cycle. The prevalence of this definition of central metabolism is, however, equivocal without experimental validation. We address this issue using a general experimental approach that combines the monitoring of transcriptional and metabolic flux changes between steady states on alternative carbon sources. This approach is investigated by using the model bacterium Pseudomonas putida with glucose, fructose, and benzoate as carbon sources. The catabolic reactions involved in the initial uptake and metabolism of these substrates are expected to show a correlated change in gene expressions and metabolic fluxes. However, there was no correlation for the reactions linking the 12 biomass precursor molecules, indicating a regulation mechanism other than mRNA synthesis for central metabolism. This result substantiates evidence for a (re)definition of central carbon metabolism including all reactions that are bound to tight regulation and transcriptional invariance. Contrary to expectations, the canonical Entner-Doudoroff and EMP pathwa...Continue Reading

References

Feb 4, 1977·Archives of Microbiology·M H SawyerR H Berman
Sep 28, 1977·Archives of Microbiology·J P Van Dijken, J R Quayle
Jan 1, 1984·Annual Review of Microbiology·J Preiss
Jan 28, 1983·European Journal of Pharmacology·A J SchreursF P Nijkamp
Aug 1, 1982·Journal of General Microbiology·J R Thayer, M L Wheelis
Jul 1, 1996·Research in Microbiology·A H Romano, T Conway
Dec 11, 1999·Nature·L H HartwellA W Murray
Oct 18, 2000·Nature·H JeongA L Barabási
Feb 24, 2001·Nature·A SchmidB Witholt
Mar 14, 2001·Journal of Biotechnology·B Kessler, B Witholt
Dec 14, 2001·Journal of Bacteriology·Marcel EmmerlingUwe Sauer
Feb 8, 2002·Microbiology·Manuel Espinosa-UrgelJuan-Luis Ramos
Jun 26, 2002·Journal of Bacteriology·Brian J KoebmannPeter R Jensen
Nov 28, 2002·Nature Genetics·John Quackenbush
Jan 22, 2003·Environmental Microbiology·José Ignacio JiménezEduardo Díaz
Feb 1, 2003·Nature Biotechnology·Lawrence P Wackett
Sep 2, 2003·Journal of Biotechnology·Robert GerstmeirBernhard J Eikmanns
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD
Jan 22, 2004·Nature Reviews. Genetics·Albert-László Barabási, Zoltán N Oltvai
Aug 28, 2004·Trends in Biotechnology·Marie Csete, John Doyle
Aug 27, 2005·BMC Bioinformatics·Nicola ZamboniUwe Sauer
Nov 1, 2005·Current Opinion in Microbiology·Bettina Siebers, Peter Schönheit
Aug 19, 2006·BMC Bioinformatics·Jing ZhaoYi-Xue Li
Apr 28, 2007·Science·Uwe SauerNicola Zamboni
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Pascale Daran-LapujadeBarbara M Bakker
Nov 17, 2010·Nature Chemical Biology·Claudia E VickersJens O Krömer

❮ Previous
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Citations

Dec 25, 2015·FEBS Open Bio·Karel OlavarriaJosé Gregório Cabrera Gomez
Jun 24, 2015·Applied Microbiology and Biotechnology·Anita Loeschcke, Stephan Thies
Sep 18, 2015·MicrobiologyOpen·Samantha S SasnowLudmilla Aristilde
Apr 11, 2020·Chembiochem : a European Journal of Chemical Biology·Manuel Nieto-Domínguez, Pablo I Nikel
Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Sep 13, 2019·Scientific Reports·Edgar A P WrightAlexander V Goltsev
Sep 18, 2020·PloS One·Gina ChaputKristen M DeAngelis
Oct 10, 2018·Environmental Microbiology Reports·Isotta D'ArrigoKatherine S Long
Sep 11, 2019·Environmental Microbiology Reports·Sarah DethlefsenBurkhard Tümmler
Feb 6, 2020·Frontiers in Bioengineering and Biotechnology·Isabel BatorLars M Blank
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Till TisoLars M Blank
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Isabel BatorLars M Blank
Jan 12, 2021·Frontiers in Bioengineering and Biotechnology·Yuqian GaoKristin E Burnum-Johnson
Apr 1, 2021·Biotechnology Advances·Maria Martin-PascualVitor A P Martins Dos Santos
Apr 3, 2021·The Science of the Total Environment·Rohan M ShahDavid J Beale

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