The Fur homologue BosR requires Arg39 to activate rpoS transcription in Borrelia burgdorferi and thereby direct spirochaete infection in mice

Microbiology
Laura I Katona

Abstract

Borrelia burgdorferi is the causative agent of Lyme disease. In B. burgdorferi, RpoS controls the expression of virulence genes needed for mammalian infection. The Fur homologue BosR regulates the transcription of rpoS and therefore BosR determines, albeit indirectly, the infection status of the spirochaete. Transcription of rpoS in B. burgdorferi is complex: rpoS can be transcribed either from an RpoD-dependent promoter to yield a long transcript or from an RpoN-dependent promoter to yield a short transcript. This study shows that BosR repressed synthesis of the long transcript while at the same time activating synthesis of the short transcript. How BosR does this is unclear. To address this, spirochaetes were engineered to express either BosR or the naturally occurring variant BosRR39K. Mice became infected by the spirochaetes expressing BosR but not by the spirochaetes expressing BosRR39K. Furthermore, the spirochaetes expressing BosR activated rpoS transcription during growth in culture whereas the spirochaetes expressing BosRR39K did not. Thus, BosR's activation of rpoS transcription somehow involves Arg39. This arginine is highly conserved in other FUR proteins and therefore other FUR proteins may also require this argini...Continue Reading

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Citations

Aug 24, 2016·Infection and Immunity·Adrienne C ShowmanMollie W Jewett
Jun 22, 2016·Infection and Immunity·Zhiming OuyangMichael V Norgard
Jul 22, 2018·Frontiers in Cellular and Infection Microbiology·Dan DrecktrahD Scott Samuels
May 2, 2019·Frontiers in Cellular and Infection Microbiology·Charlotte MasonZhiming Ouyang
Feb 2, 2021·Archives of Biochemistry and Biophysics·Emma SevillaMaría F Fillat

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