The Fusarium graminearum Histone Acetyltransferases Are Important for Morphogenesis, DON Biosynthesis, and Pathogenicity

Frontiers in Microbiology
Xiangjiu KongJie Feng

Abstract

Post-translational modifications of chromatin structure by histone acetyltransferase (HATs) play a central role in the regulation of gene expression and various biological processes in eukaryotes. Although HAT genes have been studied in many fungi, few of them have been functionally characterized. In this study, we identified and characterized four putative HATs (FgGCN5, FgRTT109, FgSAS2, FgSAS3) in the plant pathogenic ascomycete Fusarium graminearum, the causal agent of Fusarium head blight of wheat and barley. We replaced the genes and all mutant strains showed reduced growth of F. graminearum. The ΔFgSAS3 and ΔFgGCN5 mutant increased sensitivity to oxidative and osmotic stresses. Additionally, ΔFgSAS3 showed reduced conidia sporulation and perithecium formation. Mutant ΔFgGCN5 was unable to generate any conidia and lost its ability to form perithecia. Our data showed also that FgSAS3 and FgGCN5 are pathogenicity factors required for infecting wheat heads as well as tomato fruits. Importantly, almost no Deoxynivalenol (DON) was produced either in ΔFgSAS3 or ΔFgGCN5 mutants, which was consistent with a significant downregulation of TRI genes expression. Furthermore, we discovered for the first time that FgSAS3 is indispensabl...Continue Reading

References

Jul 1, 1984·Molecular and Cellular Biology·G LucchiniG R Fink
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A G Hinnebusch, G R Fink
Apr 16, 1998·Genes & Development·K Struhl
Feb 20, 1999·The Journal of Biological Chemistry·P A GrantJ L Workman
Jan 19, 2000·Nature·B D Strahl, C D Allis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 22, 2003·Current Opinion in Cell Biology·Wolfgang FischleC David Allis
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·V G ALLFREYA E MIRSKY
Jun 10, 2004·Cell·Siavash K KurdistaniMichael Grunstein
Jul 31, 2004·Annual Review of Phytopathology·Guihua Bai, Gregory Shaner
Jan 22, 2005·The Journal of Biological Chemistry·Wei-Jong ShiaJerry L Workman
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carin JansenFrank J Maier
Oct 19, 2006·The Journal of Biological Chemistry·Jessica SchneiderAli Shilatifard
Feb 17, 2007·Molecular Microbiology·You-Kyoung HanYin-Won Lee
Mar 24, 2007·Nature Reviews. Molecular Cell Biology·Kenneth K Lee, Jerry L Workman
Oct 24, 2007·Fungal Genetics and Biology : FG & B·Kye-Yong SeongH Corby Kistler
Nov 28, 2007·Eukaryotic Cell·Rafael C Prados Rosales, Antonio Di Pietro
Dec 20, 2007·Molecular Systems Biology·Hung PhamMatteo Pellegrini
Jan 29, 2008·FEMS Microbiology Reviews·Gerald BroschStefan Graessle
Aug 7, 2008·Nucleic Acids Research·Yanfei Zou, Xin Bi
Sep 16, 2008·FEMS Microbiology Letters·Ekaterina Shelest
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 6, 2009·Microbiology·Donald M GardinerJohn M Manners
Nov 6, 2009·Molecular Plant-microbe Interactions : MPMI·Donald M GardinerJohn M Manners
Nov 1, 2004·Molecular Plant Pathology·Rubella S Goswami, H Corby Kistler
Apr 13, 2011·PLoS Genetics·Benoit GuillemetteRichard J Festenstein
May 27, 2011·Applied Microbiology and Biotechnology·Ludmila V RozeJohn E Linz
Jul 30, 2011·BMC Plant Biology·Marike J Boenisch, Wilhelm Schäfer

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Citations

Jul 14, 2019·FEMS Microbiology Reviews·Jérôme Collemare, Michael F Seidl
Mar 21, 2019·Annual Review of Phytopathology·Yun ChenZhonghua Ma
Nov 3, 2020·Frontiers in Microbiology·Junzhu ChenXiaotian Huang
Apr 29, 2021·Annual Review of Phytopathology·Wende LiuXiao-Lin Chen
Sep 8, 2021·Frontiers in Microbiology·Manlin XuYucheng Chi

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Methods Mentioned

BETA
acetylation
PCR
ELISA
RNA-seq
protein assay
electrophoresis
histone acetylation
environmental stress

Key Resources (RRID) Mentioned

AB_302613
AB_2115283
AB_2722568
AB_2616593
AB_2722570
AB_2714186
AB_2722569
AB_955447

Software Mentioned

Pro
BLASTP
Plus
Statistical Analysis System
hisat2
Integrative Genomics Viewer ( IGV )
MEGA
Adobe Photoshop
Image
samtools

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