Jun 4, 2017

The fusogen AFF-1 can rejuvenate the regenerative potential of adult dendritic trees by self-fusion

Development
Veronika KravtsovBenjamin Podbilewicz

Abstract

The aging brain undergoes structural changes that affect brain homeostasis, neuronal function and consequently cognition. The complex architecture of dendritic arbors poses a challenge to understanding age-dependent morphological alterations, behavioral plasticity and remodeling following brain injury. Here, we use the PVD polymodal neurons of C. elegans as a model to study how aging affects neuronal plasticity. Using confocal live imaging of C. elegans PVD neurons, we demonstrate age-related progressive morphological alterations of intricate dendritic arbors. We show that mutations in daf-2, which encodes an insulin-like growth factor receptor ortholog, fail to inhibit the progressive morphological aging of dendrites and do not prevent the minor decline in response to harsh touch during aging. We uncovered that PVD aging is characterized by a major decline in the regenerative potential of dendrites following experimental laser dendrotomy. Furthermore, the remodeling of transected dendritic trees by AFF-1-mediated self-fusion can be restored in old animals by daf-2 mutations, and can be differentially re-established by ectopic expression of the fusion protein AFF-1. Thus, ectopic expression of the fusogen AFF-1 in the PVD and m...Continue Reading

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Citations

Mentioned in this Paper

Calcinus elegans
Cyartonema elegans
Brain Injuries
Coleonyx elegans
Insulin-Like Growth Factor I
Cestrum elegans
Clarkia unguiculata
Clathrulina elegans
Cardioglossa elegans
Pulmonary Valve Disorder

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