The Gac-Rsm and SadB signal transduction pathways converge on AlgU to downregulate motility in Pseudomonas fluorescens.

PloS One
Francisco Martínez-GraneroMarta Martín

Abstract

Flagella mediated motility in Pseudomonas fluorescens F113 is tightly regulated. We have previously shown that motility is repressed by the GacA/GacS system and by SadB through downregulation of the fleQ gene, encoding the master regulator of the synthesis of flagellar components, including the flagellin FliC. Here we show that both regulatory pathways converge in the regulation of transcription and possibly translation of the algU gene, which encodes a sigma factor. AlgU is required for multiple functions, including the expression of the amrZ gene which encodes a transcriptional repressor of fleQ. Gac regulation of algU occurs during exponential growth and is exerted through the RNA binding proteins RsmA and RsmE but not RsmI. RNA immunoprecipitation assays have shown that the RsmA protein binds to a polycistronic mRNA encoding algU, mucA, mucB and mucD, resulting in lower levels of algU. We propose a model for repression of the synthesis of the flagellar apparatus linking extracellular and intracellular signalling with the levels of AlgU and a new physiological role for the Gac system in the downregulation of flagella biosynthesis during exponential growth.

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Citations

Apr 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wook KimKevin R Foster
Jul 3, 2013·Applied and Environmental Microbiology·Sarah C SeatonStuart B Levy
Mar 7, 2014·Environmental Microbiology·Mustafa FazliTim Tolker-Nielsen
May 18, 2016·FEMS Microbiology Letters·Aroa López-SánchezFernando Govantes
Aug 5, 2016·Frontiers in Microbiology·María Isabel Ramos-GonzálezManuel Espinosa-Urgel
Sep 10, 2016·Microbiology·Stéphanie BorlandFlorence Wisniewski-Dyé
Nov 14, 2015·Molecular Microbiology·Harold A Prada-RamírezMaría-Trinidad Gallegos
Dec 21, 2017·Molecular Plant-microbe Interactions : MPMI·María-Dolores FerreiroMaría Trinidad Gallegos
Sep 20, 2017·Journal of Bacteriology·Helen YakhninPaul Babitzke
Jun 22, 2016·Journal of Bacteriology·Eric MarkelBryan Swingle
Jul 17, 2016·Applied and Environmental Microbiology·Óscar Huertas-RosalesManuel Espinosa-Urgel
Apr 3, 2015·Microbiology and Molecular Biology Reviews : MMBR·Christopher A VakulskasTony Romeo
Feb 2, 2018·Scientific Reports·Candela MurielMarta Martín
May 7, 2019·Frontiers in Microbiology·Daniel Pérez-MendozaMaría Trinidad Gallegos
Nov 7, 2019·Scientific Reports·Marie-Francoise Noirot-GrosPhilippe Noirot
Jul 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Luke P AllsoppAlain Filloux
Dec 3, 2020·Molecular Plant-microbe Interactions : MPMI·Haibi WangBrian H Kvitko
Apr 4, 2021·International Journal of Molecular Sciences·Mathilde BouteillerAnnabelle Merieau
Apr 15, 2021·Molecular Plant-microbe Interactions : MPMI
May 4, 2021·Environmental Microbiology·María-Dolores Ferreiro, María-Trinidad Gallegos
Oct 16, 2014·Journal of Proteome Research·Florian-Alexander HerbstMorten S Dueholm

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Datasets Mentioned

BETA
CP003150

Methods Mentioned

BETA
transmission electron microscopy
PCR
immunoprecipitation assay
immunoprecipitation
RNA-IP
electrophoresis

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