PMID: 3758060Sep 1, 1986Paper

The gamma 1 and gamma 2 subunits of human liver alcohol dehydrogenase. cDNA structures, two amino acid replacements, and compatibility with changes in the enzymatic properties

European Journal of Biochemistry
J O HöögH von Bahr-Lindström

Abstract

cDNA clones corresponding to two alleles of the ADH3 locus were identified by hybridization with synthetic oligodeoxyribonucleotides specific for class I human liver alcohol dehydrogenase. Sequences were determined for a 1457-nucleotide cDNA, covering the whole gamma 2-coding region, and a 1224-nucleotide cDNA, including the region coding for amino acid residues 53-374 of the gamma 1 subunit. Two amino acid replacements between the gamma 1 and gamma 2 subunits were identified. At position 349, isoleucine in gamma 1 instead of valine in gamma 2 is a conservative exchange of a superficial residue which has been ascribed no special importance. The other exchange, at position 271, arginine in gamma 1 and glutamine in gamma 2, explains differences in enzyme properties. Electrophoretically, it is consistent with the less cathodic mobility of the gamma 2 subunit. Functionally, the location of the exchange at the surface of the coenzyme-binding pocket may influence the dissociation of the reduced coenzyme.

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Citations

Jan 1, 1987·Pharmacology & Therapeutics·D W CrabbT K Li
Jan 1, 1990·Pharmacology & Therapeutics·D P Agarwal, H W Goedde
Feb 26, 1998·Alcohol·W E Lands
Feb 3, 2009·The Lancet Oncology·Nathalie Druesne-PecolloPaule Latino-Martel
May 27, 2008·Nature Genetics·Mia HashibePaul Brennan
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H JörnvallB L Vallee
Aug 1, 1989·European Journal of Biochemistry·Y MatsuoS Yokoyama
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Jul 25, 1989·Biochemistry·B A NussrallahF W Wagner
Oct 27, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Abraham Fainsod, Hadas Kot-Leibovich
Oct 6, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yehuda Shabtai, Abraham Fainsod
Apr 15, 2000·The Journal of Biological Chemistry·H A Charlier, B V Plapp
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Jan 17, 2004·Stroke; a Journal of Cerebral Circulation·Robert Y L ZeeNancy R Cook
Apr 3, 2015·Physiological Reviews·Yuri L DorokhovTatiana V Komarova

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