The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction

The Journal of Immunology : Official Journal of the American Association of Immunologists
Ryusuke YoshimiYoshiaki Ishigatsubo

Abstract

Leukocyte extravasation is an important step of inflammation, in which integrins have been demonstrated to play an essential role by mediating the interaction of leukocytes with the vascular endothelium and the subendothelial extracellular matrix. Previously, we identified an integrin-linked kinase (ILK)-binding protein affixin (beta-parvin), which links initial integrin signals to rapid actin reorganization, and thus plays critical roles in fibroblast migration. In this study, we demonstrate that gamma-parvin, one of three mammalian parvin family members, is specifically expressed in several lymphoid and monocytic cell lines in a complementary manner to affixin. Like affixin, gamma-parvin directly associates with ILK through its CH2 domain and colocalizes with ILK at focal adhesions as well as the leading edge of PMA-stimulated U937 cells plated on fibronectin. The overexpression of the C-terminal fragment containing CH2 domain or the depletion of gamma-parvin by RNA interference inhibits the substrate adhesion of MCP-1-stimulated U937 cells and the spreading of PMA-stimulated U937 cells on fibronectin. Interestingly, the overexpression of the CH2 fragment or the gamma-parvin RNA interference also disrupts the asymmetric distr...Continue Reading

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Citations

Mar 28, 2008·Journal of Cellular and Molecular Medicine·Cimona V HintonHava Karsenty Avraham
Apr 27, 2012·Rejuvenation Research·Anatoliy I YashinSvetlana V Ukraintseva
Dec 25, 2009·The EMBO Journal·Sara A WickströmReinhard Fässler
Aug 8, 2012·The Journal of Biological Chemistry·Amy L StieglerTitus J Boggon
Feb 13, 2021·Cancers·Pinelopi A NikolopoulouVassiliki Kostourou
Sep 19, 2008·Journal of Cell Science·Paul C McDonaldShoukat Dedhar
May 29, 2012·Journal of Cell Science·Moritz WidmaierReinhard Fässler
Oct 28, 2008·Current Opinion in Structural Biology·Björn SjöblomKristina Djinović-Carugo

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