PMID: 4173Feb 1, 1976

The gas chromatographic mass spectrometric determination of trifluoroacetic acid in biological fluid. Application to halothane metabolism

Biomedical Mass Spectrometry
D KarashimaT Kuhara


The methyl ester of trifluoroacetic acid was prepared by reaction with N,N'-dimethylformamide dimethylacetal and was successfully passed through a gas chromatograph. Trifluoroacetic acid was detected by the use of gas chromatography and low and high resolution gas chromatography mass spectrometry in an acidic extract of an incubation medium containing microsomes, reduced nicotinamide adenine dinucleotide phosphate, oxygen and halothane. However, trifluoroacetic acid could not be detected when nicotinamide adenine dinucleotide or oxygen was omitted from the incubation system. From these results, it was proved that halothane is oxidatively metabolized to trifluoroacetic acid by hepatic microsomes.


Aug 1, 1971·Anesthesiology·E N Cohen
Feb 1, 1972·Anesthesiology·D A BlakeH F Cascobi
Jan 1, 1958·Annual Review of Biochemistry·B B BRODIEB N LA DU
Nov 1, 1964·Biochemical Pharmacology·A STIER
May 1, 1965·Anesthesiology·R A VANDYKE, M B CHENOWETH


May 1, 1994·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Y SakaguchiS Takahashi
Jan 1, 1988·Critical Reviews in Toxicology·L S Kaminsky, J M Fraser
Aug 17, 2005·Journal of Applied Physiology·Thomas SchrickerFranco Carli
Dec 18, 2013·The Journal of Veterinary Medical Science·Tetsuya HasegawaShigeo Takenaka

Related Concepts

Chromatography, Gas-Liquid
Hydrogen-Ion Concentration
Chromatography, Gas-Liquid-Mass Spectrometry
Microsomes, Liver

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