PMID: 9194577May 27, 1997Paper

The gastrin-releasing peptide receptor is differentially coupled to adenylate cyclase and phospholipase C in different tissues

Biochimica Et Biophysica Acta
L J GarciaR T Jensen

Abstract

Recent studies suggest that in some tissues GRP receptor activation can both stimulate phospholipase C and the adenylate cyclase pathway and that activation of the latter pathway may be important in mediating some of its well-described growth effects. However, other studies suggest GRP-R may not be coupled to adenylate cyclase. To investigate this possibility, in the present study we determined the coupling of the GRP receptors to each pathway in mouse, rat, and guinea pig pancreatic acini and compared it to that in mouse Swiss 3T3 cells and human SCLC cells, all of which possess well-characterized GRP receptors. Moreover, we tested the effect of PKC activation on the ability of GRP-related peptides to increase cAMP accumulation in these tissues. Changes in cAMP levels were determined with or without IBMX present, with or without forskolin, or both to amplify small increases in cAMP. In mouse, rat and guinea pig pancreatic acini, murine Swiss 3T3 cells and human SCLC cells, GRP-related peptides caused a 600%, 500%, 250%, 300% and 60% increase, respectively, in [3H]IP with 1-3 nM causing a half-maximal effect. In murine Swiss 3T3 cells, IBMX, forskolin, and IBMX plus forskolin caused a 300%, 3500% and 10500% increase in cAMP, re...Continue Reading

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E RozengurtM Collins
May 1, 1982·The Journal of Clinical Endocrinology and Metabolism·M A GhateiS R Bloom

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Citations

Nov 19, 2003·European Journal of Biochemistry·Alberto G AndreolottiLuis J Garcia-Marin
Jul 28, 2001·Diabetes Technology & Therapeutics·R PerfettiS Busselen
Dec 7, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Irene Ramos-AlvarezR T Jensen
Aug 19, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Branimir Zogovic, Paul M Pilowsky
Mar 11, 2003·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Melvyn Davies
Mar 10, 2001·Nuclear Medicine Communications·C Van de WieleR A Dierckx

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