The gastrointestinal hormone ghrelin modulates inhibitory neurotransmission in deep laminae of mouse spinal cord dorsal horn

Endocrinology
Angela Maria VergnanoAdalberto Merighi

Abstract

Ghrelin is mainly described for its effects on feeding behavior and metabolism. However, central nervous system distribution of its receptor [type 1a GH secretagogue receptor (GHSR)] and modulation of neurotransmission in the hypothalamus suggest broader effects than originally predicted. Systemically administrated ghrelin inhibits inflammatory pain after behavioral observations. Therefore, we investigated the expression and function of type 1a GHSR in mouse spinal cord by molecular biology, biochemistry, histology, and electrophysiology. The mRNA and protein were detected in tissue extracts by RT-PCR and Western blotting. In situ, receptor mRNA and immunoreactivity were localized to cell bodies within the medial aspect of the deep dorsal horn. Patch clamp recordings on laminae IV-VI demonstrated that bath-applied ghrelin (100 nm) induced a strong increase of spontaneous gamma-aminobutyric acid/glycine-mediated current frequency (463 +/- 93% of the control) and amplitude (150 +/- 16% of the control) in about 60% of recorded neurons. Specificity of type 1a GHSR activation was confirmed by the lack of effect of the deacylated form of ghrelin (des-acyl-ghrelin) and after preincubation with the specific receptor antagonist [d-Lys(3...Continue Reading

References

Feb 13, 2001·Biochemical and Biophysical Research Communications·H HosodaK Kangawa
Jun 1, 2001·Regulatory Peptides·C Dornonville de la CourR Håkanson
May 25, 2002·The New England Journal of Medicine·David E CummingsJonathan Q Purnell
Jul 11, 2002·Histochemistry and Cell Biology·Guido RindiEnrico Solcia
Jul 18, 2002·Journal of Neuroendocrinology·M TraebertH A Schmid
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·William A BanksMark L Heiman
Nov 27, 2002·Neuropeptides·R J RodgersJ E Blundell
Mar 26, 2003·The European Journal of Neuroscience·Ekkasit KumarnsitGareth Leng
Apr 21, 2004·Nature Neuroscience·Marco van den TopDavid Spanswick
Jun 9, 2004·Frontiers in Neuroendocrinology·Márta KorbonitsAshley B Grossman
Mar 25, 2005·Physiological Reviews·Masayasu Kojima, Kenji Kangawa
Dec 2, 2005·The Journal of Comparative Neurology·Jeffrey M ZigmanJoel K Elmquist
Feb 24, 2006·Nature Neuroscience·Sabrina DianoTamas L Horvath
Jun 9, 2006·Neuropharmacology·Valeria SibiliaCarmela Netti
Jul 4, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Owais ChaudhriSteve Bloom
Nov 2, 2007·ANZ Journal of Surgery·Donald Simpson

❮ Previous
Next ❯

Citations

Oct 16, 2009·Molecular Interventions·Laura S Stone, Derek C Molliver
Feb 3, 2012·Physiological Reviews·Hanns Ulrich ZeilhoferGonzalo E Yévenes
Apr 25, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·XiaFang ChenZhenJuan He
Jan 23, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Francesco FerriniChiara Salio
Aug 28, 2010·International Journal of Peptides·Johannes D Veldhuis, Cyril Y Bowers
Mar 29, 2014·Rheumatology International·Diogo HomannNeiva Leite
Mar 10, 2011·Journal of Chemical Neuroanatomy·Adalberto MerighiLaura Lossi
Jun 22, 2010·Neuroscience Letters·Ensari GuneliMukaddes Gumustekin
Jun 21, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B AvauI Depoortere
Feb 2, 2013·Arthritis and Rheumatism·Maria MorellMario Delgado
Mar 12, 2013·European Journal of Pain : EJP·D HomannN Leite
Aug 28, 2015·Molecular Neurobiology·Sareh PandamoozLeila Dargahi
Dec 18, 2013·Neurobiology of Disease·María MorellMario Delgado
May 9, 2014·Frontiers in Neurology·Carmela GiordanoGiuseppe Biagini
Jun 24, 2008·European Journal of Pain : EJP·Sarah PatersonRoman Rukwied
Jan 31, 2017·International Journal of Molecular Sciences·Ken HowickHarriët Schellekens
Jan 8, 2019·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Jijun XuJianguo Cheng
Jun 21, 2020·Scientific Reports·Mathieu MeleineGuillaume Gourcerol

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.