The GC and window-averaged DNA curvature profile of secondary metabolite gene cluster in Aspergillus fumigatus genome

Applied Microbiology and Biotechnology
Jin Hwan Do, Satoru Miyano

Abstract

An immense variety of complex secondary metabolites is produced by filamentous fungi including Aspergillus fumigatus, a main inducer of invasive aspergillosis. The identification of fungal secondary metabolite gene cluster is essential for the characterization of fungal secondary metabolism in terms of genetics and biochemistry through recombinant technologies such as gene disruption and cloning. Most of the prediction methods for secondary metabolite gene cluster severely depend on homology searches. However, homology-based approach has intrinsic limitation to unknown or novel gene cluster. We analyzed the GC and window-averaged DNA curvature profile of 26 secondary metabolite gene clusters in the A. fumigatus genome to find out potential conserved features of secondary metabolite gene cluster. Fifteen secondary metabolite gene clusters showed a conserved pattern in window-averaged DNA curvature profile, that is, the DNA regions including secondary metabolic signature genes such as polyketide synthase, nonribosomal peptide synthase, and/or dimethylallyl tryptophan synthase consisted of window-averaged DNA curvature values lower than 0.18 and these DNA regions were at least 20 kb. Forty percent of secondary metabolite gene clus...Continue Reading

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BolshoyE N Trifonov
Feb 11, 1982·Nucleic Acids Research·W KabschE N Trifonov
Jul 22, 1995·Molecular & General Genetics : MGG·F Kempken
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C UeguchiT Mizuno
Aug 1, 1993·Computer Applications in the Biosciences : CABIOS·E S ShpigelmanA Bolshoy
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R Goddard, A Burt
Aug 9, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Ohyama
Aug 22, 2001·Trends in Microbiology·J P Latgé
Jul 19, 2002·FEBS Letters·Ryoiti Kiyama, Edward N Trifonov
Oct 12, 2004·Journal of Biomolecular Structure & Dynamics·S Hosid, A Bolshoy
Dec 3, 2005·Nature Reviews. Microbiology·Nancy P KellerJoan W Bennett
Apr 17, 2007·PLoS Pathogens·Robyn M PerrinNancy P Keller

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Citations

Apr 3, 2012·Indian Journal of Clinical Biochemistry : IJCB·Preetida J BhetariyaSarma P Usha
Apr 11, 2012·International Journal of Molecular Sciences·Feng-Biao Guo, Wen Wei
Oct 18, 2011·Genetics and Molecular Research : GMR·W Wei, F-B Guo

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