The GCR2 gene family is not required for ABA control of seed germination and early seedling development in Arabidopsis.

PloS One
Jianjun GuoJin-Gui Chen

Abstract

The plant hormone abscisic acid (ABA) regulates diverse processes of plant growth and development. It has recently been proposed that GCR2 functions as a G-protein-coupled receptor (GPCR) for ABA. However, the structural relationships and functionality of GCR2 have been challenged by several independent studies. A central question in this controversy is whether gcr2 mutants are insensitive to ABA, because gcr2 mutants were shown to display reduced sensitivity to ABA under one experimental condition (e.g. 22 degrees C, continuous white light with 150 micromol m(-2) s(-1)) but were shown to display wild-type sensitivity under another slightly different condition (e.g. 23 degrees C, 14/10 hr photoperiod with 120 micromol m(-2) s(-1)). It has been hypothesized that gcr2 appears only weakly insensitive to ABA because two other GCR2-like genes in Arabidopsis, GCL1 and GCL2, compensate for the loss of function of GCR2. In order to test this hypothesis, we isolated a putative loss-of-function allele of GCL2, and then generated all possible combinations of mutations in each member of the GCR2 gene family. We found that all double mutants, including gcr2 gcl1, gcr2 gcl2, gcl1 gcl2, as well as the gcr2 gcl1 gcl2 triple mutant displayed wi...Continue Reading

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Citations

Jul 10, 2014·Journal of Experimental Botany·Chao MeiDa-Peng Zhang
Mar 3, 2010·Annual Review of Plant Biology·Sean R CutlerSuzanne R Abrams
Apr 21, 2009·Nature Chemical Biology·Aaron SantnerMark Estelle
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Jul 9, 2011·Plant Cell Reports·Adam ColvilleBrian Miki
Jul 9, 2009·Journal of Experimental Botany·Jianjun GuoJin-Gui Chen
Jun 26, 2009·Nature·Aaron Santner, Mark Estelle
Mar 27, 2021·The Plant Journal : for Cell and Molecular Biology·Lennard van der WoudeMartijn van Zanten
Jun 3, 2021·Plants·Rafael Ruiz-PartidaJorge Lozano-Juste

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Methods Mentioned

BETA
PCR
genotyping
chips

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