The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation

Molecular Microbiology
J van der PlasP Weisbeek

Abstract

The gene encoding plastocyanin (petE1) from Anabaena sp. PCC 7937 was isolated using two sets of mixed oligonucleotide hybridization probes derived from conserved regions in the protein. Plastocyanin is encoded as a preprotein of 139 amino acids. The amino-terminal extension of 34 residues has all the characteristics of a signal peptide and is probably involved in translocation of preplastocyanin over the thylakoid membrane. The level of the petE1 mRNA, a single transcript of about 740 bases, was found to be severely reduced under conditions of Cu2+ deficiency. The petE1 gene was transferred to the genome of Synechococcus sp. PCC 7942, which did not appear to contain a structural gene for plastocyanin itself. The integrated gene becomes expressed at the transcriptional level, regardless of the amount of Cu2+ available.

References

Jul 25, 1975·Journal of Molecular Biology·A DugaiczykH M Goodman
Feb 8, 1979·Biochimica Et Biophysica Acta·K K HoC Gomez-Lojero
Oct 28, 1976·Nature·A Aitken
Jan 1, 1977·International Journal of Bio-medical Computing·A M Fails, J M Verlander
Jan 16, 1987·Cell·G Brawerman
Jan 1, 1986·Current Genetics·C RotherR G Herrmann
Aug 1, 1987·Journal of Cutaneous Pathology·N G Ordóñez, N A Samaan
Jun 16, 1986·European Journal of Biochemistry·R J SimpsonM Murata
Jun 18, 1985·European Journal of Biochemistry·T Sako
Dec 1, 1974·The Biochemical Journal·J Kelly, R P Ambler
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Jan 1, 1970·Molecular & General Genetics : MGG·S V Shestakov, N T Khyen
May 1, 1984·Analytical Biochemistry·J Meinkoth, G Wahl
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Mar 25, 1983·Nucleic Acids Research·L DenteR Cortese
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Nov 25, 1983·Nucleic Acids Research·T Sako, N Tsuchida
Jul 11, 1984·Nucleic Acids Research·M E Watson
Jan 1, 1983·Molecular & General Genetics : MGG·N Tomioka, M Sugiura
Jan 11, 1984·Nucleic Acids Research·C Queen, L J Korn
Sep 15, 1983·Journal of Molecular Biology·J M Guss, H C Freeman
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B J MazurR Haselkorn
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S E Curtis, R Haselkorn
Apr 1, 1980·Plant Physiology·D J DavisA S Pietro
Apr 1, 1988·Photosynthesis Research·S Smeekens, P Weisbeek

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Citations

Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
Feb 1, 1990·Trends in Biochemical Sciences·S SmeekensC Robinson
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y TakeshimaH Hagiwara
Jun 11, 1990·Nucleic Acids Research·C J Howe, T P Wallace
Dec 1, 1993·The Veterinary Quarterly·M J WeijtensM H Biesheuvel
Jan 1, 2002·The Arabidopsis Book·Ben ScheresLiam Dolan
Jan 27, 2021·Proceedings of the National Academy of Sciences of the United States of America·Raquel García-CañasLuis López-Maury

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