The gene overexpressed in polycythemia rubra vera, PRV-1, and the gene encoding a neutrophil alloantigen, NB1, are alleles of a single gene, CD177, in chromosome band 19q13.31

Transfusion
Lorraine CaruccioD F Stroncek

Abstract

PRV-1 mRNA is overexpressed by neutrophils from polycythemia vera patients and is homologous to NB1 a gene overexpressed in reactive neutrophilia. These investigations were designed to confirm searches of genome databases suggesting that PRV-1 and NB1 are alleles of the same gene, CD177, and confirm a pseudogene adjacent to CD177. Methods included polymerase chain reaction (PCR), cloning, sequencing, and fluorescent hybridization studies. The coding region of PRV-1 was PCR-amplified from human fetal RNA, cloned, and used to screen the RPCI-11 bacterial artificial chromosome (BAC) library. Five BACs were reactive with the PRV-1 probe. PCR analysis of the BACs with primers encompassing PRV-1 exons, containing four known single-nucleotide polymorphisms, followed by sequencing rendered amplicons identical to PRV-1 in all five BACs. Analysis of all five by restriction digestion yielded fragments possible only if both the gene and the pseudogene are present. End sequencing of the BACs localized them to the same chromosome region. G-banding and fluorescence in situ hybridization at the 400- and 850-band levels of resolution mapped one BAC to chromosome band 19q13.2 and sublocalized the BAC to band 19q13.31, respectively. PRV-1 and NB1...Continue Reading

References

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Oct 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Luciana TeofiliLuigi Maria Larocca

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Citations

Sep 28, 2007·Current Opinion in Hematology·David F Stroncek
Oct 13, 2006·Journal of Translational Medicine·Michael Bennett, David F Stroncek
Mar 1, 2013·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Jun Abe, Akio Matsuda
Nov 2, 2014·Protein & Cell·Qing XieWeizhou Zhang
Nov 3, 2007·Experimental Hematology·Jaroslav JelinekVahid Afshar-Kharghan
Oct 15, 2013·American Journal of Clinical Pathology·Howard J MeyersonAnna Balog
Nov 2, 2006·Journal of Leukocyte Biology·Susanne BauerThomas Hellmark
Nov 30, 2019·Transfusion Medicine·Kanokpol SiriphanthongOytip Nathalang
Jun 21, 2007·The Journal of Biological Chemistry·Ulrich J H SachsSentot Santoso
Jun 1, 2017·The Journal of Experimental Medicine·Claudia Eulenberg-GustavusRalph Kettritz
Dec 2, 2018·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Brigitte Katharina Flesch, Angelika Reil

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