The gene slyA of Salmonella typhimurium is required for destruction of M cells and intracellular survival but not for invasion or colonization of the murine small intestine.

Infection and Immunity
J J DanielsW Goebel

Abstract

Recent studies have shown that Salmonella typhimurium invades the M cells of Peyer's patches (PP) of the murine ileum. The slyA gene of S. typhimurium has also recently been reported to affect virulence of this pathogen in mice and survival in macrophages. We therefore compared the effect on PP tissue of four strains of S. typhimurium: a wild-type strain, two slyA insertion mutants, and a recombinant S. typhimurium derivative carrying multiple copies of slyA. Invasion assays performed 2 and 7 days after orogastric infection revealed significantly lower numbers of bacteria of the slyA mutants and of the SlyA-overproducing strain in PP than of the wild type. However, similar numbers of bacteria of all strains were still present in the lumen of the small intestine after these times. Invasion assays of PP tissue after 90-min ileal loop infection yielded comparable numbers of bacteria of all strains in PP. Transmission and scanning electron microscopy of PP tissue after ileal loop infection demonstrated that the two slyA mutants and the SlyA-overproducing strain were able to attach to, induce membrane ruffling of, and invade M cells in a way morphologically and quantitatively similar to that of the wild type. In contrast to the wild...Continue Reading

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Citations

Oct 6, 2000·Annual Review of Microbiology·P A Cotter, V J DiRita
May 31, 2003·Journal of Bacteriology·Valia A NorteJeffrey Green
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian K CoombesB Brett Finlay
Oct 7, 2015·Frontiers in Microbiology·Marc Erhardt, Petra Dersch
Dec 3, 1999·Immunological Reviews·J C SirardJ P Kraehenbuhl
Sep 15, 2000·Journal of Bacteriology·C J HerbelinT S Whittam
Apr 20, 2002·Current Opinion in Infectious Diseases·Eduardo A. Groisman, Chakib Mouslim
Sep 26, 2007·Molecular Microbiology·James K LithgowJeffrey Green
Apr 10, 2002·Microbiology and Immunology·Aki KanekoHirofumi Danbara
Jun 23, 2009·Journal of Bacteriology·M Manjurul HaqueShinji Tsuyumu
Oct 18, 2000·FEMS Microbiology Letters·J J DanielsW Goebel
Aug 24, 1999·Infection and Immunity·P R WatsonT S Wallis
Jun 12, 2010·Microbes and Infection·Amit LahiriDipshikha Chakravortty
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
May 21, 2003·International Journal of Medical Microbiology : IJMM·M Ann Clark, Mark A Jepson

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