The generation and characterization of LMP2-specific CTLs for use as adoptive transfer from patients with relapsed EBV-positive Hodgkin disease

Journal of Immunotherapy
Catherine M BollardCliona M Rooney

Abstract

Cellular adoptive immunotherapy for virus-associated malignant disease is an attractive strategy, since viral antigens provide targets for specific T lymphocytes. In Epstein-Barr virus (EBV)-positive Hodgkin disease (HD), a limited number of EBV-encoded antigens such as the latent membrane antigens (LMP) 1 and 2 are expressed on the malignant Reed-Sternberg cells. The authors aimed to generate cytotoxic T lymphocytes (CTLs) from patients with relapsed HD by specifically targeting LMP2A. Patients with relapsed HD have highly immunosuppressive tumors and have been heavily pretreated with cytotoxic agents. As a result, monocytes and lymphocytes are numerically reduced and functionally impaired. Approaches using dendritic cells (DCs) as the sole antigen-presenting cell to expand LMP2-specific CTL lines in vitro have proved impractical. The authors now show how small amounts of patient peripheral blood can be used to produce DCs expressing LMP2 after Ad5F35 transduction, and how an initial reactivation of LMP2-specific CTLs can be followed by stimulation with lymphoblastoid cell lines overexpressing LMP2 from the same vector. Large numbers of LMP2-specific cytotoxic lymphocytes are produced that contain both CD4+ and CD8+ T cells (f...Continue Reading

References

Dec 25, 1983·Journal of Molecular Biology·R M Sweet, D Eisenberg
Jan 1, 1995·The Journal of General Virology·P BussonN Raab-Traub
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P TrivediG Winberg
Oct 24, 2000·Journal of Virology·D M Shayakhmetov, A Lieber
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T LuftJ Cebon
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Pauline MeijJaap M Middeldorp
Sep 19, 2002·International Journal of Cancer. Journal International Du Cancer·Riccardo GavioliMaria G Masucci

❮ Previous
Next ❯

Citations

Jan 25, 2008·Current Treatment Options in Oncology·Kevin A DavidAndrew M Evens
May 27, 2009·Immunology and Cell Biology·Viviana P LutzkyDenis J Moss
Dec 22, 2004·The Journal of Experimental Medicine·Catherine M BollardHelen E Heslop
Jun 12, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J I CohenE S Jaffe
May 10, 2006·Immunology and Cell Biology·Siok-Keen TeyHelen E Heslop
Nov 30, 2006·Annual Review of Immunology·Ann M LeenAaron E Foster
Jun 22, 2006·International Journal of Hematology·Alana A Kennedy-NasserCliona M Rooney
Feb 14, 2013·Drug Design, Development and Therapy·Lu ZhangHai-Qiang Mai
Dec 2, 2014·Progress in Retinal and Eye Research·Jonas KuiperTimothy Radstake
Jul 18, 2006·Biochemical and Biophysical Research Communications·Ying PanYi Zeng
Dec 29, 2004·Leukemia & Lymphoma·Stephen GottschalkCliona M Rooney
Jan 8, 2016·Leukemia Research·Li ZhouXiao-Tong Song
Mar 24, 2010·Pediatric Clinics of North America·Nabil AhmedCrystal L Mackall
Sep 16, 2008·Best Practice & Research. Clinical Haematology·Catherine M BollardHelen E Heslop
Mar 4, 2009·Update on Cancer Therapeutics·John Craddock, Helen E Heslop
Jun 10, 2006·Hematology/oncology Clinics of North America·Cassian Yee
Dec 21, 2012·Pediatric Blood & Cancer·Kara M KellyUNKNOWN COG Hodgkin Lymphoma Committee
Dec 24, 2005·Pathology·Stephen R Larsen, John E J Rasko
Nov 21, 2007·Critical Reviews in Oncology/hematology·Yvette L Kasamon, Richard F Ambinder
Nov 19, 2013·Cytotherapy·Usanarat AnurathapanJuan F Vera
Nov 5, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sharon LamCatherine Bollard
Oct 27, 2010·Advances in Hematology·Claudio Fozza, Maurizio Longinotti
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michael P SeilerBrendan Lee
Dec 18, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Catherine M BollardCliona M Rooney
Nov 6, 2010·Expert Opinion on Pharmacotherapy·Bharti Rathore, Marshall E Kadin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.