The generation and evaluation of two panels of epitope-matched mouse IgG1, IgG2a, IgG2b and IgG3 antibodies specific for Plasmodium falciparum and Plasmodium yoelii merozoite surface protein 1-19 (MSP1(19)).

Experimental Parasitology
Jaime R Adame-GallegosRichard J Pleass

Abstract

Murine immunoglobulin G (IgG) plays an important role in mediating protective immune responses to malaria. We still know relatively little about which IgG subclasses protect against this disease in mouse models, although IgG2a and IgG2b are considered to be the most potent and dominate in successful passive transfer experiments in rodent malarias. To explore the mechanism(s) by which the different mouse IgG subclasses may mediate a protective effect, we generated mouse IgG1, IgG2a, IgG2b and IgG3 specific for the C-terminal 19-kDa region of Plasmodium falciparum merozoite surface protein 1 (PfMSP1(19)), and to the homologous antigen from Plasmodium yoelii (P. yoelii), both major targets of protective immune responses. This panel of eight IgGs bound antigen with an affinity comparable to that seen for their epitope-matched parental monoclonal antibodies (mAbs) from which they were derived, although for reasons of yield, we were only able to explore the function of mouse IgG1 recognizing PfMSP1(19) in detail, both in vitro and in vivo. Murine IgG1 was as effective as the parental human IgG from which it was derived at inducing NADPH-mediated oxidative bursts and degranulation from neutrophils. Despite showing efficacy in in vitro...Continue Reading

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Citations

Feb 13, 2016·Frontiers in Microbiology·Alena JandaArturo Casadevall
Apr 16, 2015·Parasitology Research·Martha Legorreta-HerreraJorge Morales-Montor
Aug 15, 2014·Journal of Leukocyte Biology·Stephanie KapelskiRolf Fendel
Mar 24, 2020·Journal of Leukocyte Biology·Michael YangDenise L Faustman
Feb 6, 2020·Experimental and Therapeutic Medicine·Hongbo ChenLongxin Qiu
Jan 9, 2019·Frontiers in Immunology·Elizabeth H AitkenStephen J Rogerson
May 22, 2018·Bioinformatics and Biology Insights·Daniela Droppa-AlmeidaFrancine Ferreira Padilha
Jul 3, 2017·International Journal for Parasitology·Danika L HillDanny W Wilson

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Methods Mentioned

BETA
PCR
surface
transgenic
chip
Biosensor
ELISA
ELISAs
surface plasmon resonance
glycosylation

Software Mentioned

BIAsimulation

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