The generation and functional characterization of induced pluripotent stem cells from human intervertebral disc nucleus pulposus cells

Oncotarget
Yanxia ZhuXiaoping Yu

Abstract

Disc degenerative disease (DDD) is believed to originate in the nucleus pulposus (NP) region therefore, it is important to obtain a greater number of active NP cells for the study and therapy of DDD. Human induced pluripotent stem cells (iPSCs) are a powerful tool for modeling the development of DDD in humans, and have the potential to be applied in regenerative medicine. NP cells were isolated from DDD patients following our improved method, and then the primary NP cells were reprogramed into iPSCs with Sendai virus vectors encoding 4 factors. Successful reprogramming of iPSCs was verified by the expression of surface markers and presence of teratoma. Differentiation of iPSCs into NP-like cells was performed in a culture plate or in hydrogel, whereby skin fibroblast derived-iPSCs were used as a control. Results demonstrated that iPSCs derived from NP cells displayed a normal karyotype, expressed pluripotency markers, and formed teratoma in nude mice. NP induction of iPSCs resulted in the expression of NP cell specific matrix proteins and related genes. Non-induced NP derived-iPSCs also showed some NP-like phenotype. Furthermore, NP-derived iPSCs differentiate much better in hydrogel than that in a culture plate. This is a nove...Continue Reading

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Citations

Mar 11, 2018·Stem Cell Research & Therapy·Ruhang TangLori A Setton
Aug 29, 2020·International Journal of Molecular Sciences·Clara Sanjurjo-RodríguezSilvia Díaz-Prado
Sep 20, 2018·Current Stem Cell Research & Therapy·Kaishun XiaChengzhen Liang

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Methods Mentioned

BETA
PCR

Software Mentioned

AxioVision

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