PMID: 9178813May 16, 1997Paper

The genes for gonadotropin-releasing hormone and its receptor are expressed in human breast with fibrocystic disease and cancer

International Journal of Cancer. Journal International Du Cancer
M L KottlerR Counis

Abstract

While gonadotropin-releasing hormone (GnRH) or GnRH receptor (GnRHR) have been reported to exist in tissues other than brain and pituitary, there is no report concerning co-expression of GnRH and GnRHR in human breast tissues. To address this question, we have examined whether mRNA for GnRH as well as GnRHR was present in different human breast samples, by employing the reverse transcription-polymerase chain reaction (RT-PCR) protocol followed by Southern blotting of the PCR products. Coexpression of GnRH and GnRHR genes was further confirmed by dot blot hybridization using appropriate [32P]-labeled probes. We thus tested fibrocystic breast (4 cases), invasive ductal carcinomas (6 cases) and 1 adjacent non-neoplastic tissue. GnRHR and GnRH mRNAs were found in all actin-positive samples including malignant as well as nonmalignant tissues. Quantitative determinations of mRNA did not reveal significant differences between malignant and non-malignant breast samples for either GnRH or GnRHR gene expression. Our data show that neither gene was overexpressed in the breast cancer samples compared with normal breast tissue. Since GnRH agonists inhibit breast epithelial cell growth, the presence of GnRHR mRNA suggests that GnRH may speci...Continue Reading

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Citations

Sep 17, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Kirsty Cleverly, T John Wu
Aug 19, 2007·Breast Cancer Research : BCR·Djura PiersmaEls M J J Berns
Mar 9, 2013·PloS One·Sudarshan Gadadhar, Anjali A Karande
Apr 3, 2001·Expert Opinion on Investigational Drugs·P LimontaR M Moretti
Mar 3, 2012·The Breast : Official Journal of the European Society of Mastology·María del Pilar Carrera-GonzálezJosé Manuel Martínez-Martos
Jun 15, 2011·International Journal of Pharmaceutics·Marie PribylovaTomas Vanek
Sep 15, 2005·Journal of Neuroendocrinology·J P HapgoodK Ronacher
Oct 31, 2008·The FEBS Journal·Lydia W T Cheung, Alice S T Wong
Mar 11, 2011·Annals of the New York Academy of Sciences·Anne-Laure SchangJean-Noël Laverrière
Nov 26, 2008·Molecular and Cellular Endocrinology·Yewei XingWilliam E Rainey
Mar 21, 2006·Cancer Letters·Sarah KrausRony Seger
Feb 5, 2010·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Anna G JankowskaProf Jerzy B Warchol
May 9, 2000·Journal of Endocrinological Investigation·A ParadisoS J Reshkin
Oct 22, 2019·Frontiers in Oncology·Maira Huerta-ReyesArturo Aguilar-Rojas
Jun 29, 2005·Animal Reproduction Science·N RamakrishnappaP C K Leung

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