PMID: 2110141May 1, 1990Paper

The genes for three xylan-degrading activities from Bacteroides ovatus are clustered in a 3.8-kilobase region

Journal of Bacteriology
T R Whitehead, R B Hespell

Abstract

Genes coding for three xylan-degrading activities, xylanase, xylosidase, and arabinosidase, were simultaneously cloned from the colonic anaerobic organism Bacteriodes ovatus. The genes for the three enzymes were located on a 3.8-kilobase EcoRI genomic insert and were cloned by using plasmid pUC18. All three activities were expressed in Escherichia coli JM83, and all were cell associated. Expression of the xylanase gene was independent from expression of the xylosidase and arabinosidase genes, whereas expression of the latter two genes appeared to be coordinated. Restriction endonuclease analysis of the arabinosidase and xylosidase genes and partial purification of these enzyme activities from E. coli suggested that these activities were catalyzed by a bifunctional protein or two proteins of very similar molecular weight. All three enzyme activities were regulated in B. ovatus in response to the carbon source used for growth. This is the first report of the cloning and expression of B. ovatus genes.

References

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Citations

Mar 15, 2003·Microbiology and Immunology·Ohmi Ueda, Fuminobu Yoshimura
Feb 18, 2011·Bioengineered Bugs·David A Berry
Apr 16, 2016·AMB Express·Ghulam MustafaAmer Jamil
Oct 1, 1990·Journal of Dairy Science·R B Hespell, T R Whitehead
May 1, 1992·Molecular & General Genetics : MGG·P E Vercoe, K Gregg
Jan 12, 2011·Molecular Microbiology·Dylan DoddIsaac K O Cann
May 17, 2013·Annals of the Royal College of Surgeons of England·Z Z R Hamady
Jan 1, 1993·FEMS Microbiology Reviews·J A Thomson
May 28, 2005·Canadian Journal of Microbiology·Hidenori HayashiYoshimi Benno
Jan 1, 1996·Biotechnology Advances·N N Kumar, D N Deobagkar
Jun 23, 2000·Enzyme and Microbial Technology·N Belfaquih, M J Penninckx
Feb 2, 2010·Applied and Environmental Microbiology·S KoikeY Kobayashi
Jun 22, 2010·Applied and Environmental Microbiology·María Cristina RavanalJaime Eyzaguirre
Sep 1, 1992·Applied and Environmental Microbiology·J WeaverA A Salyers
Apr 1, 1992·Applied and Environmental Microbiology·R B Hespell, P J O'bryan
Aug 17, 2010·Applied and Environmental Microbiology·Shengguo ZhaoZhongtang Yu

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