PMID: 9531633Jun 6, 1998Paper

The genes lmbB1 and lmbB2 of Streptomyces lincolnensis encode enzymes involved in the conversion of L-tyrosine to propylproline during the biosynthesis of the antibiotic lincomycin A

Archives of Microbiology
D NeusserW Piepersberg

Abstract

The genes lmbA,B1,B2 in the lincomycin A production gene cluster of Streptomyces lincolnensis were shown to form a common transcription unit with the promoter located directly upstream of lmbA. The proteins LmbB1 (mol. mass, 18 kDa) and LmbB2 (mol. mass 34 kDa), when over-produced together in Escherichia coli, brought about enzyme activities for the specific conversion of both L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) to a yellow-colored product. The LmbB1 protein alone catalyzed the conversion of L-DOPA, but not of L-tyrosine. The purified LmbB1 protein showed a Km for L-DOPA of 258.3 microM. The L-tyrosine converting activity could not been demonstrated in vitro. The preliminary interpretation of these data suggests that the protein LmbB1 is an L-DOPA extradiol-cleaving 2,3-dioxygenase and that the protein LmbB2, either alone or in accord with LmbB1, represents an L-tyrosine 3-hydroxylase. This sequence of putative oxidation reactions on L-tyrosine seems to represent a new pathway different from the ones catalyzed by mammalian L-tyrosine hydroxylases or the wide-spread tyrosinases. The protein LmbA seemed not to be involved in this process. The labile, yellow-colored product from L-DOPA could not be converted to a ...Continue Reading

Citations

Nov 1, 2003·Applied Microbiology and Biotechnology·J Spízek, T Rezanka
Feb 4, 2010·Journal of the American Chemical Society·Christopher T Walsh, Michael A Fischbach
Sep 10, 2004·European Journal of Biochemistry·Jitka NovotnáJaroslav Spízek
Mar 10, 2009·Applied and Environmental Microbiology·Wei LiBarbara Gerratana
Mar 10, 2009·Applied and Environmental Microbiology·Wei LiBarbara Gerratana
Jan 26, 2013·Chemical Society Reviews·Chia-I LinHung-wen Liu
Jun 15, 2010·Medicinal Research Reviews·Barbara Gerratana
Jun 23, 2007·Chemistry & Biology·Yunfeng HuBrian O Bachmann
Oct 30, 2013·Chembiochem : a European Journal of Chemical Biology·Lucie NajmanováJiří Janata
Mar 20, 2018·Applied Microbiology and Biotechnology·Bingbing HouHuizhan Zhang
Mar 19, 2021·Journal of the American Chemical Society·Yifan WangAimin Liu
Jul 30, 2021·Biochemistry·Alexander M GoldbergLarryn W Peterson
Dec 24, 2019·Chemical Reviews·Jason B Hedges, Katherine S Ryan
Nov 7, 2021·Scientific Reports·Mihir V ShahColin Scott

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