The genes of the coactivator TIF2 and the corepressor SMRT are primary 1alpha,25(OH)2D3 targets

The Journal of Steroid Biochemistry and Molecular Biology
Thomas W DunlopCarsten Carlberg

Abstract

The complex of the receptor for the hormone 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)), Vitamin D(3) receptor (VDR), the retinoid X receptor (RXR) and a 1alpha,25(OH)(2)D(3) response element (VDRE) is considered to be the molecular switch for nuclear 1alpha,25(OH)(2)D(3) signaling. In the presence of ligand the VDR-RXR complex interacts with coactivator (CoA) proteins that in turn contact components of the basal transcriptional machinery resulting in an enhanced transcription of 1alpha,25(OH)(2)D(3) target genes. In the absence of ligand the VDR remains bound to the DNA and interacts with corepressor (CoR) proteins that are involved in gene silencing activity. We treated MCF-7 breast cancer cells with 1alpha,25(OH)(2)D(3) for increasing amounts of time, extracted mRNA and screened by real-time PCR the members of the p160 CoA and NCoR CoR families. We find that of the p160 coactivators, only TIF2 was responsive to 1alpha,25(OH)(2)D(3). Similarly SMRT but not NCoR1 gene transcription was sensitive to 1alpha,25(OH)(2)D(3) treatment. In silico analysis revealed that both TIF2 and SMRT promoters have substantial numbers of VDREs compared to the promoters of the other family members. These VDREs are formed by direct repea...Continue Reading

References

Feb 18, 1993·Nature·C CarlbergW Hunziker
Jul 23, 1998·Critical Reviews in Eukaryotic Gene Expression·C Carlberg, P Polly
Jul 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P PollyC Carlberg
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L J Burke, A Baniahmad
Feb 28, 2003·Developmental Biology·Stephan P TenbaumAria Baniahmad

❮ Previous
Next ❯

Citations

Feb 10, 2009·International Journal of Colorectal Disease·Petros D GrivasHaralabos Kalofonos
Jun 8, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jorge Rojas-RiveraJesús Egido
Aug 10, 2010·Biochimica Et Biophysica Acta·Juha M MatilainenSami Väisänen
May 27, 2014·Frontiers in Physiology·Moray J Campbell
Jan 31, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jiri VrbaJitka Ulrichova
Oct 23, 2014·Journal of Cellular Physiology·Mark D LongMoray J Campbell
Jun 14, 2005·American Journal of Physiology. Renal Physiology·Adriana S DussoEduardo Slatopolsky
Jun 5, 2014·International Journal of Cancer. Journal International Du Cancer·James L Thorne, Moray J Campbell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.