The genetic basis of antibody production: a single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse

European Journal of Immunology
M SiekevitzM L Gefter

Abstract

A nucleic acid probe specific for heavy chains bearing the cross-reactive idiotype (Id) associated with the anti-p-azophenylarsonate response of strain A mice has been prepared. Analysis of arsonate-binding Id+ hybridoma cell lines has revealed that all of them contain the same germ-line VH gene rearranged to the JH2 segment. An Id+ hybridoma which is unable to bind arsonate utilized the same VH gene, but it has apparently rearranged to the JH4 segment. Id- cell lines contain other rearranged VH genes. Analysis of DNa of strain A mice revealed that there is apparently only one germ line gene that can give rise to Id+ heavy chains. Since the Id is expressed as a large collection (greater than 50) of related but nonidentical heavy chain sequences, we conclude that their diversity is the result of a somatic mutation process. Analysis of a single hybridoma cell line (45-59) reveals that somatic mutation can operate on an Id-encoding gene and result in an antigen-binding molecule that has lost all of its Id determinants. Further analysis of the genome of strain A mice has revealed the presence of germ-line genes differing from the Id-encoding gene by at least 8 base pairs. These genes, however, apparently do not contribute to the an...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WahlG R Stark
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Collins, B Hohn
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S TonegawaR Schuller
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J A LaskinP D Gottlieb
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S TonegawaW Gilbert
Apr 1, 1973·The Journal of Experimental Medicine·L L Pawlak, A Nisonoff
Jul 2, 1973·European Journal of Biochemistry·M Gross-BellardP Chambon
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I M Cesari, M Weigert
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Dec 1, 1982·European Journal of Immunology·M SiekevitzA Marshak-Rothstein
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Marshak-RothsteinM L Gefter

❮ Previous
Next ❯

Citations

Dec 4, 2001·Journal of Immunological Methods·Behnaz Parhami-SerenMichael N Margolies
Feb 24, 2000·Molecular Immunology·G GololobovS Paul
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R I NearM L Gefter
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T Manser, M L Gefter
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S OhnoT Matsunaga
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K S HathcockR J Hodes
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R T BoydP D Gottlieb
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F RobbinsA Nisonoff
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L WysockiM L Gefter
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J SharonM Ptashne
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M M SeidmanK H Kraemer
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I Sanz, J D Capra
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J KippsD A Carson
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R R PollockM D Scharff
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Tutter, R Riblet
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M B LascombeR J Poljak
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J DurdikE Selsing
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B C Lubahn, H M Reisner
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H LiuL J Wysocki
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A M GiustiT Manser
Jan 1, 1984·The Journal of Experimental Medicine·R M PerlmutterL Hood
Jul 1, 1984·The Journal of Experimental Medicine·M SlaouiJ Urbain
Jun 1, 1985·The Journal of Experimental Medicine·M E Boersch-SupanT Imanishi-Kari
Jul 1, 1986·The Journal of Experimental Medicine·S HabaA Nisonoff
Feb 1, 1987·The Journal of Experimental Medicine·C T LutzJ M Davie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.