PMID: 2114185Mar 1, 1990Paper

The genetic transfer, heredity and phenotypic expression of plasmid RP4::Mu cts genes in Bacillus cereus

Biulleten' eksperimental'noĭ biologii i meditsiny
N V TimakovaB N Il'iashenko

Abstract

The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid PR4::Mu cts 62 genes for antibiotic resistance and temperature sensitivity. Production of the bacteriophage Mu cts 62 particles was not registered in the bacillary transcipient cells. The plasmid RP4::Mu cts 62 genes were localized in the chromosome of Bac. cereus 682 transcipient by the blot-hybridization technique with 32P-labelled DNA of the bacteriophage Mu cts 62 and the plasmid PR4. The transcipient of Bac. cereus 682 has the donor properties and transfers the RP4::Mu cts 62 genes to recipient cells of Bac. cereus DSM 318 with the frequency of 10(-6)-10(-7). The expression and transfer of the gram-negative plasmid genes in gram-positive bacterial cells are discussed.

References

Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M GonzálezB C Carlton

❮ Previous
Next ❯

Citations

Oct 1, 1985·Biulleten' eksperimental'noĭ biologii i meditsiny·N M EvdokimovaA G Skavronskaia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
S Chang, S N Cohen
Molekuliarnaia genetika, mikrobiologiia i virusologiia
V V DanileĭchenkoB N Il'iashenko
© 2021 Meta ULC. All rights reserved