Abstract
Mortality for infants on the heart transplant waitlist remains unacceptably high, and available mechanical circulatory support is suboptimal. Our goal is to demonstrate the feasibility of utilizing genetically engineered pig (GEP) heart as a bridge to allotransplantation by transplantation of a GEP heart in a baboon. Four baboons underwent orthotopic cardiac transplantation from GEP donors. All donor pigs had galactosyl-1,3-galactose knocked out. Two donor pigs had human complement regulatory CD55 transgene and the other 2 had human complement regulatory CD46 and thrombomodulin. Induction immunosuppression included thymoglobulin, and anti-CD20. Maintenance immunosuppression was rapamycin, anti-CD-40, and methylprednisolone. One donor heart was preserved with University of Wisconsin solution and the other three with del Nido solution. All baboons weaned from cardiopulmonary bypass. B217 received a donor heart preserved with University of Wisconsin solution. Ventricular arrhythmias and depressed cardiac function resulted in early death. All recipients of del Nido preserved hearts easily weaned from cardiopulmonary bypass with minimal inotropic support. B15416 and B1917 survived for 90 days and 241 days, respectively. Histopatholo...Continue Reading
References
Jan 28, 2009·Circulation·Christopher S D AlmondT P Singh
Nov 3, 2009·The Journal of Thoracic and Cardiovascular Surgery·Anke Katharina FurckHans-Heiner Kramer
Aug 22, 2015·Xenotransplantation·Hayato IwaseDavid K C Cooper
Jan 21, 2017·The Journal of Thoracic and Cardiovascular Surgery·James R Butler, A Joseph Tector
Dec 12, 2017·British Medical Bulletin·D K C CooperA J Tector
Mar 25, 2018·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·William T MahleUNKNOWN Pediatric Heart Network Investigators
Jul 17, 2018·The Annals of Thoracic Surgery·Raheel RizwanDavid Morales
Oct 12, 2018·Pediatric Cardiology·David ClevelandDavid K C Cooper
Dec 7, 2018·Nature·Matthias LänginJan-Michael Abicht
Mar 1, 2019·The Annals of Thoracic Surgery·David L S MoralesUNKNOWN Pedimacs Investigators
Jun 1, 2019·Journal of Inflammation·Juan LiHayato Iwase
Oct 4, 2019·The Annals of Thoracic Surgery·Qi LiDavid C Cleveland
Dec 20, 2019·Xenotransplantation·Matthias LänginJan-Michael Abicht
May 5, 2020·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Theo M M H de ByOliver Miera
Sep 19, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Daniel J HurstWayne Paris
Oct 7, 2020·Pediatric Transplantation·Luz A PadillaWaldemar F Carlo
Dec 19, 2020·Xenotransplantation·David K C Cooper, David C Cleveland