The genome of bacteriophage phiKZ of Pseudomonas aeruginosa

Journal of Molecular Biology
Vadim V MesyanzhinovGuido Volckaert

Abstract

Bacteriophage phiKZ is a giant virus that efficiently infects Pseudomonas aeruginosa strains pathogenic to human and, therefore, it is attractive for phage therapy. We present here the complete phiKZ genome sequence and a preliminary analysis of its genome structure. The 280,334 bp genome is a linear, circularly permutated and terminally redundant, A+T-rich double-stranded DNA molecule. The phiKZ DNA has no detectable sequence homology to other viruses and microorganisms, and it does not contain NotI, PstI, SacI, SmaI, XhoI, and XmaIII endonuclease restriction sites. The genome has 306 open reading frames (ORFs) varying in size from 50 to 2237 amino acid residues. According to the orientation of transcription, ORFs are apparently organized into clusters and most have a clockwise direction. The phiKZ genome also encodes six tRNAs specific for Met (AUG), Asn (AAC), Asp (GAC), Leu (TTA), Thr (ACA), and Pro (CCA). A putative promoter sequence containing a TATATTAC block was identified. Most potential stem-loop transcription terminators contain the tetranucleotide UUCG loops. Some genes may be assigned as phage-encoded RNA polymerase subunits. Only 59 phiKZ gene products exhibit similarity to proteins of known function from a divers...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C TuerkI Tinoco
Aug 10, 1989·Nature·O BerghM Heldal
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A ShubM Belfort
Jan 10, 1986·Nucleic Acids Research·R Staden
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F K ChuM Belfort
Jun 1, 1984·Canadian Journal of Microbiology·V N KrylovE A Khrenova
Oct 15, 1984·Journal of Molecular Biology·D EisenbergR Wall
Jan 1, 1993·Archives of Virology·V N KrylovV Z Akhverdian
Jan 1, 1993·Annual Review of Biochemistry·A M Lambowitz, M Belfort
Jan 1, 1993·Bio Systems·M Borodovsky, J McIninch
Jan 1, 1995·Virus Genes·E KutterV Mesyanzhinov
Nov 1, 1996·Current Biology : CB·B R Levin, R V Tauxe
Mar 28, 1997·Journal of Molecular Biology·C MonodH M Krisch
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 5, 1997·Science·J Engel
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V LazarevicD Karamata
Nov 20, 1998·The Journal of Infection·J AliskyN Troitsky
Apr 16, 1998·Science·M C SmithR W Hendrix
Dec 10, 1998·Nucleic Acids Research·K HofmannA Bairoch
Jan 19, 1999·Advances in Virus Research·H W Ackermann
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·R W HendrixG F Hatfull
Jun 15, 1999·Bioinformatics·S Kurtz, C Schleiermacher
Sep 11, 1999·Nucleic Acids Research·J Besemer, M Borodovsky
Nov 5, 1999·Annual Review of Microbiology·J L Van Etten, R H Meints
Nov 5, 1999·Journal of Molecular Biology·M Gottesman
Dec 11, 1999·Nucleic Acids Research·A Bairoch, R Apweiler
Dec 11, 1999·Nucleic Acids Research·A BatemanE L Sonnhammer

❮ Previous
Next ❯

Citations

Mar 18, 2010·Molecular & Cellular Proteomics : MCP·Julie A ThomasStephen C Hardies
Nov 25, 2003·Nucleic Acids Research·Ryan MillsMark Borodovsky
Jul 12, 2011·Nucleic Acids Research·Darius Kazlauskas, Ceslovas Venclovas
Jan 17, 2012·Science·Weimin WuAlasdair C Steven
Mar 5, 2005·Applied and Environmental Microbiology·Hsiao-Chuan ChangShu-Fen Weng
Dec 11, 2007·Journal of Bacteriology·Takashi YoshidaHiroyuki Ogata
Oct 19, 2004·Journal of Bacteriology·Sandra Chibani-ChennoufiHarald Brüssow
Jan 24, 2006·Journal of Bacteriology·Tony KwanJerry Pelletier
Sep 29, 2004·Journal of Virology·Lisa M KattenhornBenedikt M Kessler
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Nov 16, 2010·Canadian Journal of Microbiology·Lingyan LiShiru Jia
Oct 28, 2009·BMC Microbiology·Rob LavigneAndrew M Kropinski
Feb 28, 2007·Virology Journal·Philip SerwerStephen C Hardies
Mar 16, 2007·Virology Journal·Philip Serwer
Nov 17, 2010·PloS One·Jun ZhuangLianhui Xie
Apr 18, 2013·PloS One·Eugenijus SimoliūnasRolandas Meškys
Jul 17, 2010·Future Microbiology·Pieter-Jan Ceyssens, Rob Lavigne
Sep 18, 2013·Antimicrobial Agents and Chemotherapy·Marine HenryLaurent Debarbieux
Oct 23, 2015·Nucleic Acids Research·Maria YakuninaLeonid Minakhin
Oct 7, 2015·Bacteriophage·Hans-W Ackermann
Apr 6, 2006·FEMS Microbiology Reviews·Christoph Weigel, Harald Seitz
May 27, 2014·Frontiers in Pharmacology·Santi M MandalOctavio L Franco
Aug 6, 2005·Journal of Molecular Biology·Andrei FokineVadim V Mesyanzhinov
Jan 22, 2005·Virology·Jianbin WangSongnian Hu
Sep 14, 2007·Structure·Andrei FokineMichael G Rossmann
Feb 9, 2017·Frontiers in Microbiology·Colin ButtimerAidan Coffey
Nov 18, 2017·Genome Announcements·Ian N D EsplinJulianne H Grose

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.