DOI: 10.1101/517466Jan 11, 2019Paper

The genome of C57BL/6J "Eve", the mother of the laboratory mouse genome reference strain

BioRxiv : the Preprint Server for Biology
Vishal Kumar SarsaniLaura Reinholdt

Abstract

Isogenic laboratory mouse strains are used to enhance reproducibility as individuals within a strain are essentially genetically identical. For the most widely used isogenic strain, C57BL/6, there is also a wealth of genetic, phenotypic, and genomic data, including one of the highest quality reference genomes (GRCm38.p6). However, laboratory mouse strains are living reagents and hence genetic drift occurs and is an unavoidable source of accumulating genetic variability that can have an impact on reproducibility over time. Nearly 20 years after the first release of the mouse reference genome, individuals from the strain it represents (C57BL/6J) are at least 26 inbreeding generations removed from the individuals used to generate the mouse reference genome. Moreover, C57BL/6J is now maintained through the periodic reintroduction of mice from cryopreserved embryo stocks that are derived from a single breeder pair, aptly named C57BL/6J Adam and Eve. To more accurately represent the genome of today's C57BL/6J mice, we have generated a de novo assembly of the C57BL/6J Eve genome (B6Eve) using high coverage, long-read sequencing, optical mapping, and short-read data. Using these data, we addressed recurring variants observed in previou...Continue Reading

Related Concepts

Aves
DNA, Complementary
Genes
Genome
Inbreeding
Laboratory
Laboratory mice
Structure
Molecular Assembly/Self Assembly
Genetic Drift

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