The genome sequence of Pseudoplusia includens single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses

BMC Genomics
Saluana R CraveiroMaria Elita B Castro

Abstract

Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX - Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may...Continue Reading

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Citations

Feb 6, 2016·BMC Genomics·Daniel M P Ardisson-AraújoJosé L C Wolff
Sep 19, 2014·PLoS Genetics·Gaelen R BurkeMichael R Strand
Nov 4, 2016·Viruses·Christopher Noune, Caroline Hauxwell
May 3, 2020·Neotropical Entomology·D R Sosa-GómezB M Ribeiro
Dec 10, 2016·Genome Announcements·Saluana R CraveiroMaria Elita B Castro

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Datasets Mentioned

BETA
KJ631622
EU682272
EU401913
AF325155

Methods Mentioned

BETA
restriction digest
electrophoresis

Software Mentioned

SignalP
Mauve
Geneious
LBDotView
InterProScan
ORF Finder
MrBayes
Artemis
ProtTest
Geneious R6

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