The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens

Nature Biotechnology
Soon Ho HongJae Jong Kim

Abstract

The rumen represents the first section of a ruminant animal's stomach, where feed is collected and mixed with microorganisms for initial digestion. The major gas produced in the rumen is CO(2) (65.5 mol%), yet the metabolic characteristics of capnophilic (CO(2)-loving) microorganisms are not well understood. Here we report the 2,314,078 base pair genome sequence of Mannheimia succiniciproducens MBEL55E, a recently isolated capnophilic Gram-negative bacterium from bovine rumen, and analyze its genome contents and metabolic characteristics. The metabolism of M. succiniciproducens was found to be well adapted to the oxygen-free rumen by using fumarate as a major electron acceptor. Genome-scale metabolic flux analysis indicated that CO(2) is important for the carboxylation of phosphoenolpyruvate to oxaloacetate, which is converted to succinic acid by the reductive tricarboxylic acid cycle and menaquinone systems. This characteristic metabolism allows highly efficient production of succinic acid, an important four-carbon industrial chemical.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jan 1, 1995·Methods in Cell Biology·A FavelloR K Wilson
Sep 5, 1997·Science·F R BlattnerY Shao
May 16, 1998·Genome Research·D GordonP Green
Oct 31, 1998·Gene·S L SalzbergJ F Tomb
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jan 27, 1999·Bioinformatics·S R Eddy
Feb 24, 1999·International Journal of Systematic Bacteriology·M V GuettlerM K Jain
Nov 11, 1999·Nucleic Acids Research·A L DelcherS L Salzberg
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·B J MayV Kapur
Dec 26, 2001·Nucleic Acids Research·Minoru KanehisaAkihiro Nakaya
Apr 8, 2003·Trends in Biotechnology·Nathan D PriceBernhard O Palsson
Oct 7, 2004·Macromolecular Bioscience·Sang Y LeeSi J Park
May 18, 2011·The Veterinary Clinics of North America. Food Animal Practice·Thomas H Herdt, Brent Hoff

❮ Previous
Next ❯

Citations

Jul 5, 2007·Applied Microbiology and Biotechnology·James B McKinlayJ Gregory Zeikus
Mar 15, 2008·Applied Microbiology and Biotechnology·Sang Yup LeeYu-Sin Jang
Apr 2, 2008·Applied Microbiology and Biotechnology·Hyohak SongSang Yup Lee
Sep 3, 2010·Applied Microbiology and Biotechnology·Stephanie HuhnKay Marin
Jul 16, 2011·Applied Microbiology and Biotechnology·Stefanie Kind, Christoph Wittmann
Nov 21, 2007·Applied Biochemistry and Biotechnology·Lata AgarwalRajendra K Saxena
Jun 18, 2005·Current Opinion in Biotechnology·Irina Borodina, Jens Nielsen
Jun 1, 2005·Trends in Biotechnology·Sang Yup LeeTae Yong Kim
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·D P MorgaviC J Newbold
Oct 4, 2012·Animal : an International Journal of Animal Bioscience·D P MorgaviG T Attwood
Jun 14, 2013·Animal : an International Journal of Animal Bioscience·S C LeahyG T Attwood
Sep 3, 2011·Nature Protocols·Jan SchellenbergerBernhard Ø Palsson
Sep 28, 2007·Natural Product Reports·Stefano DonadioMargherita Sosio
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Trude HandalThierry Fosse
Jul 10, 2007·Applied and Environmental Microbiology·Yu-Sin JangKyu Young Kang
Jan 19, 2010·Applied and Environmental Microbiology·Jeong Wook LeeSang Yup Lee
Nov 20, 2012·Applied and Environmental Microbiology·Jun Hyoung LeeSun Chang Kim
Mar 7, 2006·Applied and Environmental Microbiology·Sang Jun LeeSang Yup Lee
Apr 17, 2008·Journal of Biomedicine & Biotechnology·Pan-Gyu KimKiejung Park
Oct 14, 2006·BMC Evolutionary Biology·Rosemary J RedfieldJohn He Nash
Oct 6, 2010·BMC Genomics·Paulraj K LawrenceRoger E Bumgarner
Dec 4, 2012·The HUGO Journal·Umashankar VetrivelSudarsanam Dorairaj
Mar 17, 2010·Current Opinion in Microbiology·Keith E J TyoJens Nielsen
Jun 28, 2008·Trends in Biotechnology·Jin Hwan ParkHyun Uk Kim
Jan 27, 2007·Trends in Biotechnology·Keith E TyoGregory N Stephanopoulos
Sep 1, 2015·Bioprocess and Biosystems Engineering·Shashi Kant BhatiaYung-Hun Yang
Aug 30, 2008·Biotechnology and Bioengineering·Sang Yup LeeKwang S Jung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.