The genome-wide rate and spectrum of spontaneous mutations differ between haploid and diploid yeast

Proceedings of the National Academy of Sciences of the United States of America
Nathaniel P SharpSarah P Otto

Abstract

By altering the dynamics of DNA replication and repair, alternative ploidy states may experience different rates and types of new mutations, leading to divergent evolutionary outcomes. We report a direct comparison of the genome-wide spectrum of spontaneous mutations arising in haploids and diploids following a mutation-accumulation experiment in the budding yeast Saccharomyces cerevisiae Characterizing the number, types, locations, and effects of thousands of mutations revealed that haploids were more prone to single-nucleotide mutations (SNMs) and mitochondrial mutations, while larger structural changes were more common in diploids. Mutations were more likely to be detrimental in diploids, even after accounting for the large impact of structural changes, contrary to the prediction that mutations would have weaker effects, due to masking, in diploids. Haploidy is expected to reduce the opportunity for conservative DNA repair involving homologous chromosomes, increasing the insertion-deletion rate, but we found little support for this idea. Instead, haploids were more susceptible to SNMs in late-replicating genomic regions, resulting in a ploidy difference in the spectrum of substitutions. In diploids, we detect mutation rate v...Continue Reading

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Citations

Jun 15, 2019·Yeast·Ciaran Gilchrist, Rike Stelkens
Jan 18, 2020·Annual Review of Food Science and Technology·B GibsonV Vidgren
Feb 12, 2020·Nature Ecology & Evolution·Piaopiao Chen, Jianzhi Zhang
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Apr 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ziyue GaoMolly Przeworski
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Dec 30, 2020·PloS One·Christian Schulz, Eivind Almaas
Oct 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yang SuiThomas D Petes
Feb 7, 2021·Global Change Biology·Wing Yan ChanMadeleine J H van Oppen
Jan 28, 2021·FEMS Microbiology Reviews·Aleeza C Gerstein, Nathaniel P Sharp
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Nov 5, 2021·Genome Biology·Ioannis A PapaioannouMichael Knop
Dec 16, 2021·Genome Biology and Evolution·Anna FijarczykChristian R Landry

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