The genomes of Crithidia bombi and C. expoeki, common parasites of bumblebees

PloS One
Paul Schmid-HempelStefan Zoller

Abstract

Trypanosomatids (Trypanosomatidae, Kinetoplastida) are flagellated protozoa containing many parasites of medical or agricultural importance. Among those, Crithidia bombi and C. expoeki, are common parasites in bumble bees around the world, and phylogenetically close to Leishmania and Leptomonas. They have a simple and direct life cycle with one host, and partially castrate the founding queens greatly reducing their fitness. Here, we report the nuclear genome sequences of one clone of each species, extracted from a field-collected infection. Using a combination of Roche 454 FLX Titanium, Pacific Biosciences PacBio RS, and Illumina GA2 instruments for C. bombi, and PacBio for C. expoeki, we could produce high-quality and well resolved sequences. We find that these genomes are around 32 and 34 MB, with 7,808 and 7,851 annotated genes for C. bombi and C. expoeki, respectively-which is somewhat less than reported from other trypanosomatids, with few introns, and organized in polycistronic units. A large fraction of genes received plausible functional support in comparison primarily with Leishmania and Trypanosoma. Comparing the annotated genes of the two species with those of six other trypanosomatids (C. fasciculata, L. pyrrhocoris...Continue Reading

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Citations

Sep 13, 2019·MSphere·Evgeny GerasimovVyacheslav Yurchenko
Aug 4, 2019·Insects·Joseph Belsky, Neelendra K Joshi
Jan 5, 2019·Parasitology Research·Kleber de Sousa PereiraIvan Meeus
May 14, 2019·Frontiers in Cellular and Infection Microbiology·Qiushi LiuTatsuhiko Kadowaki
Aug 29, 2021·Pathogens·Antonio NanettiGiovanni Cilia
Oct 20, 2018·Chemical Reviews·Vern L Schramm

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Datasets Mentioned

BETA
PRJEB21108

Methods Mentioned

BETA
glucosylation
glycosylation

Software Mentioned

Gblocks
Blast2GO
OMA browser
runAssembly
- Assembler
PhyML
HMMSCAN
Orthologous Matrix ( OMA
SNAP
SyMap

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