The geometric pattern of a pillared substrate influences the cell-process distribution and shapes of fibroblasts

Micron : the International Research and Review Journal for Microscopy
Wen-Ta SuChuan-Ding Lin

Abstract

Fibroblasts alter their shape, direction of movement, cytoskeleton arrangement, and focal contact when placed upon square array pillars. We prepared pillars of 1 microm diameter, separated by 3 microm, and having 1, 5, and 10 microm heights using substrates displaying identical surface chemistry. When cells seeded initially onto the tops of the pillars, fibroblasts subsequently were immobilized in situ by several pillars that visibly protruded through, but did not pierce, the cell bodies. The cytoplasma then migrated outward with long straight lamella along the interval of the pillars and formed several discrete attachment zones at their side walls - the value of their form index (FI) was as high as 35 - which altered the cellular shape entirely. Most of the cells interacted with the pillar substrate by spreading preferentially in a particular direction, but some of them had the ability to undergo coincident two-direction (x and y) migration; right-angle turn orientations led to the growth of dramatic cellular morphologies. Interestingly, this fibroblast's behavior variation was gradually in proportion to the pillar height of substrate. Our results confirm that cellular migration and cellular shape are both strongly affected by...Continue Reading

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Citations

Dec 17, 2009·Journal of Materials Science. Materials in Medicine·Patricia M DavidsonKarine Anselme
Jul 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Chuang-Yu LinWen-Ta Su
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Li-Chong XuChristopher A Siedlecki
May 1, 2013·Journal of Biomedical Materials Research. Part a·Chuang-Yu LinWen-Ta Su
Dec 29, 2012·Journal of Colloid and Interface Science·Barbara CorteseGiuseppe Gigli
Dec 1, 2009·Biomaterials·Li Buay KohSubbu S Venkatraman
Dec 8, 2009·Journal of Biomedical Materials Research. Part a·Wen-Ta SuLi-Tzu Li
Jun 24, 2017·ACS Applied Materials & Interfaces·Lun PengXiaohua Zhang
May 7, 2009·Nanotechnology·Raya SorkinYael Hanein
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·K AnselmeM Bigerelle
Aug 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Yu-Jing ChiuJiun-Tai Chen

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