The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression

Scientific Reports
Ana Carina Nogueira VasconcelosGoro Yoshizaki

Abstract

Since the late 19th century, the Amazon species Colossoma macropomum (tambaqui) has been exploited commercially and the climate change has contributed to decline in tambaqui numbers. Although germ cell cryopreservation and transplantation can help preserve the species' genetic resources semipermanently, its germ cell behavior has not been analyzed to date. In this study, we isolated the tambaqui's dead end gene (dnd) homolog (tdnd) and used it as a molecular marker for germ cells to obtain basic information essential for transplantation. The amino acid sequence showed 98% similarity and 53% identity with the zebrafish dnd. Phylogenetic analysis and the presence of consensus motifs known for dnd revealed that tdnd encodes the dnd ortholog and its transcript is detectable only in the testes and ovaries, showing a strong positive signal in oocytes and spermatogonia. The tambaqui possesses, at least, three different transcripts of tdnd which show dissimilar expression profile in undifferentiated and sexually mature animals, suggesting that they play distinct roles in germline development and they may influence the choice of donors for the cell transplantation study.

References

Feb 9, 1978·Nature·W Gilbert
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, J W Zimmermann
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, M R Avarbock
Mar 18, 1999·Nature Genetics·A Herbert, A Rich
Mar 1, 2000·Molecular Reproduction and Development·G YoshizakiT Takeuchi
Sep 14, 2000·American Journal of Human Genetics·S M Mount
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K I IwamiY Yoshikai
Jan 14, 2004·Nature Structural & Molecular Biology·Jesus GarciaJosep Rizo
Mar 5, 2004·Journal of Proteome Research·Alissa ReschChristopher Lee
Jun 15, 2004·Current Opinion in Structural Biology·Liana F LareauSteven E Brenner
Sep 2, 2004·Nature Genetics·Heebal KimJurg Ott
Dec 21, 2004·Nucleic Acids Research·Changning LiuRunsheng Chen
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Krasimir SlanchevErez Raz
Mar 24, 2005·Journal of the National Cancer Institute. Monographs·Kyle E Orwig, Stefan Schlatt
Jul 28, 2005·Cell and Tissue Research·Derek J McLean
Feb 16, 2006·Reproduction, Fertility, and Development·J R Hill, I Dobrinski
Mar 2, 2006·Genome Biology·Christine A WellsSean M Grimmond
Dec 13, 2006·Nucleic Acids Research·Eddo KimGil Ast
Dec 25, 2007·Cell·Martijn KeddeReuven Agami
Oct 22, 2008·Developmental Biology·Kellee R Siegfried, Christiane Nüsslein-Volhard
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Feb 26, 2009·Cell·Phillip A Sharp
Jul 7, 2009·Gene Expression Patterns : GEP·Lixiu LiuYunhan Hong
Jul 18, 2009·Current Opinion in Genetics & Development·Elliott W Abrams, Mary C Mullins
Jan 30, 2010·Nature·Timothy W Nilsen, Brenton R Graveley
Jul 3, 2010·Science China. Life Sciences·HongYan XuYunHan Hong
Aug 23, 2012·Gene·Olga KelemenStefan Stamm
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
May 24, 2014·Nature Reviews. Immunology·Susan CarpenterKatherine A Fitzgerald
Mar 24, 2015·Biomedical Reports·Yan WangXiao-Zhong Wang
Apr 13, 2017·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhi-Hui SunJian-Fang Gui
May 5, 2017·Molecular Biology and Evolution·Vincent LefortOlivier Gascuel

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Methods Mentioned

BETA
transgenic
electrophoresis
gene knock-down
PCR

Software Mentioned

MAFFT
Smart Model Selection ( SMS )
PhyML

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