The gibberellin 13-oxidase that specifically converts gibberellin A9 to A20 in Tripterygium wilfordii is a 2-oxoglutarate-dependent dioxygenase

Planta
Yifeng ZhangWei Gao

Abstract

A novel GA13-oxidase ofTripterygium wilfordii, TwGA13ox, is a 2-oxoglutarate-dependent dioxygenase. It specifically catalyzes the conversion of GA9to GA20, but not GA4to GA1. Gibberellins (GAs) play essential roles in plant growth and development. Previous characterization of GA20- and GA3-oxidases yielded a large number of genetic elements that can interconvert different GAs. However, enzymes that catalyze the 13-hydroxylation step are rarely identified. Here, we report that the GA13-oxidase of Tripterygium wilfordii, TwGA13ox, is a 2-oxoglutarate-dependent dioxygenase instead of reported cytochrome P450 oxygenases, among 376 differential proteins in comparative proteomics. Phylogenetic analysis showed that the enzyme resides in its own independent branch in the DOXC class. Unexpectedly, it specifically catalyzes the conversion of GA9 to GA20, but not GA4 to GA1. Contrary to the previous research, TwGA13ox transcriptional expression was upregulated ~ 146 times by exogenous application of methyl jasmonate (MeJA). RNAi targeting of TwGA13ox in T. wilfordii led to an 89.9% decrease of triptolide, a diterpenoid epoxide with extensive anti-inflammatory and anti-tumor properties. In subsequent MeJA supplementation experiments, tript...Continue Reading

References

May 20, 1999·Critical Reviews in Biochemistry and Molecular Biology·J D Newman, J Chappell
May 3, 2002·Journal of Plant Growth Regulation·Jake MacMillan
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oliver LauleMarkus Lange
Sep 15, 2005·Planta·Nigel E J ApplefordPeter Hedden
Jan 5, 2008·Annual Review of Plant Biology·Shinjiro Yamaguchi
Jun 13, 2008·The Plant Journal : for Cell and Molecular Biology·Kosuke KaiBun-ichi Shimizu
Dec 15, 2010·Developmental Cell·Xingliang HouHao Yu
Jan 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi MagomeShinjiro Yamaguchi
Feb 28, 2013·Development·Jean-Michel Davière, Patrick Achard
Mar 20, 2013·Phytochemistry·Maria João Pimenta LangeTheo Lange
Feb 20, 2014·The Plant Journal : for Cell and Molecular Biology·Yosuke KawaiMasaharu Mizutani
Jun 19, 2017·Current Opinion in Plant Biology·Alexandra M ShigenagaCristiana T Argueso

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Citations

Apr 29, 2020·Plant & Cell Physiology·Theo Lange, Maria João Pimenta Lange
Jun 9, 2020·Frontiers in Plant Science·Juan HeGuodong Wang
Jul 12, 2020·Plant & Cell Physiology·Peter Hedden
Nov 20, 2020·The Plant Journal : for Cell and Molecular Biology·Daichi WatanabeMasatoshi Nakajima
May 18, 2020·Seminars in Cell & Developmental Biology·Jorge Hernández-GarcíaMiguel A Blázquez

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Datasets Mentioned

BETA
GA13ox

Methods Mentioned

BETA
transgenic
PCR
transfection
gene knockdown

Software Mentioned

MEGA
InterproScan
ClustalW

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