The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system

Scientific Reports
Drew StenesenHelmut Krämer

Abstract

The Drosophila Ncc69 gene encodes a Na+-K+-2Cl--cotransporter (NKCC) that is critical for regulating intra- and extracellular ionic conditions in different tissues. Here, we show that the Ncc69 transporter is necessary for fly vision and that its expression is required non-autonomously in glia to maintain visual synaptic transmission. Flies mutant for Ncc69 exhibit normal photoreceptor depolarization in response to a light pulse but lack the ON and OFF-transients characteristic of postsynaptic responses of lamina neurons, indicating a failure in synaptic transmission. We also find that synaptic transmission requires the Ncc69 regulatory kinases WNK and Fray in glia. The ERG phenotype is associated with a defect in the recycling of the histamine neurotransmitter. Ncc69 mutants exhibit higher levels of the transport metabolite carcinine in lamina cartridges, with its accumulation most intense in the extracellular space. Our work reveals a novel role of glial NKCC transporters in synaptic transmission, possibly through regulating extracellular ionic conditions.

References

Aug 1, 1994·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·B A StewartC F Wu
Dec 1, 1996·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·J MelzigE Buchner
Sep 3, 2002·The Journal of Comparative Neurology·Arnd RichardtBernhard T Hovemann
Aug 6, 2003·The Journal of Biological Chemistry·Arnd RichardtBernhard T Hovemann
Nov 25, 2004·Neuroscience·P Kofuji, E A Newman
Nov 22, 2005·PLoS Genetics·John R TrueJianyong Li
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
May 29, 2007·Progress in Neurobiology·Ann E StuartIan A Meinertzhagen
Mar 28, 2009·Neuron·Peter BlaesseKai Kaila
Apr 17, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Johnna DohertyMarc R Freeman
Oct 16, 2009·American Journal of Physiology. Cell Physiology·Qifei SunKelly G Ten Hagen
Jan 30, 2010·Progress in Neurobiology·Tara N Edwards, Ian A Meinertzhagen
Mar 26, 2010·Metabolic Brain Disease·Arumugam R Jayakumar, Michael D Norenberg
Apr 3, 2010·Cold Spring Harbor Protocols·Richard M PartonIlan Davis
Jul 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matti Weckström, Simon Laughlin
Dec 24, 2011·Cold Spring Harbor Protocols·Tobias StorkMarc R Freeman
Mar 1, 2012·Neurochemical Research·Nanna Macaulay, Thomas Zeuthen
Aug 24, 2012·American Journal of Physiology. Cell Physiology·Aylin R RodanChou-Long Huang
Feb 6, 2013·PloS One·Atsushi Sato, Hiroshi Shibuya
Mar 20, 2013·Molecular Aspects of Medicine·Hermann Koepsell
Jun 26, 2013·EMBO Reports·Ekatherina SeryshevaAndreas Jenny
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ratna ChaturvediHong-Sheng Li
May 8, 2014·Science Signaling·Alexander T PialaElizabeth J Goldsmith
Jul 17, 2014·Science Signaling·Dario R AlessiKristopher T Kahle
Sep 23, 2014·Nature Reviews. Neuroscience·Kai KailaJuha Voipio
Jul 6, 2015·Trends in Molecular Medicine·Kristopher T KahleEric Delpire
Dec 30, 2015·Nature Neuroscience·Stefanie Robel, Harald Sontheimer
Mar 1, 2016·Cell Reports·Ratna ChaturvediHong-Sheng Li
Jul 17, 2016·The Biochemical Journal·Abinash C MistryRobert S Hoover
Jan 31, 2017·Glia·Malte C KremerUlrike Gaul

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Citations

Oct 28, 2019·The Journal of Cell Biology·Hailun LiAaron DiAntonio
May 5, 2020·The Journal of Cell Biology·Katy L H Marshall-PhelpsDavid A Lyons
Sep 1, 2020·PLoS Genetics·Maureen M SampsonDavid E Krantz
Jan 14, 2021·American Journal of Physiology. Cell Physiology·John M PleinisAylin R Rodan

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