PMID: 9428795Jan 15, 1998Paper

The glucocorticoid receptor regulates the binding of C/EPBbeta on the alpha-1-acid glycoprotein promoter in vivo

DNA and Cell Biology
G F SavoldiD Di Lorenzo

Abstract

A complex interaction between the Glucocorticoid Receptor (GR), C/EBPbeta, and other transcription factors activate the Alpha-1 Acid Glycoprotein (AGP) promoter in HTC(JZ-1) rat hepatoma culture cells. This effect is mediated by the so-called Steroid Responsive Unit (SRU) of the AGP promoter that contains several binding sites for C/EBP transcription factors, some of which overlap with the Glucocorticoid Responsive Element (GRE). Our in vivo footprinting experiments revealed that the GRE- and the C/EBP-binding sites were already occupied glucocorticoid dependently in HTC(JZ-1) cells 10 min after dexamethasone administration (10(-6) M). Furthermore, local changes in the chromatine structure shown by the appearance of DNAse I hypersensitive sites (HS sites) also took place. These changes were probably dependent on a tissue-specific organization of the chromatine at the SRU because they were not detectable in a different glucocorticoid-responsive cell line (PC12) that did not express AGP. Here, we have also shown that withdrawal of dexamethasone or addition of the anti-glucocorticoid RU486 were able to revert the pattern induced by dexamethasone in vivo. The disappearance of the protected region and the hypersensitive sites, typic...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K D Carr, H Richard-Foy
May 1, 1990·Endocrine Reviews·M A Carson-JuricaB W O'Malley
Jan 1, 1989·Annals of the New York Academy of Sciences·H BaumannG P Jahreis
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L VanniceG M Ringold
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·M TrussM Beato
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C A BeckD P Edwards
Dec 15, 1995·Cell·D J MangelsdorfR M Evans

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Citations

Nov 4, 2000·Biochimica Et Biophysica Acta·T Bratt
Jun 15, 2007·Molecular and Cellular Endocrinology·Andrew R Clark
Oct 19, 2004·Biochimica Et Biophysica Acta·Onard J L M SchoneveldWouter H Lamers
Mar 1, 2012·Cellular Signalling·René HuberKorbinian Brand
Apr 20, 2010·Molecular and Cellular Endocrinology·Agnes E Coutinho, Karen E Chapman
Nov 4, 2000·Biochimica Et Biophysica Acta·T FournierD Porquet

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