The glucocorticoid receptor represses cyclin D1 by targeting the Tcf-beta-catenin complex

The Journal of Biological Chemistry
Sachiko TakayamaBeatrice D Darimont

Abstract

The ability of glucocorticoids (GCs) to regulate cell proliferation plays an important role in their therapeutic use. The canonical Wnt pathway, which promotes the proliferation of many cancers and differentiated tissues, is an emerging target for the actions of GCs, albeit existing links between these signaling pathways are indirect. By screening known Wnt target genes for their ability to respond differently to GCs in cells whose proliferation is either positively or negatively regulated by GCs, we identified c-myc, c-jun, and cyclin D1, which encode rate-limiting factors for G(1) progression of the cell cycle. Here we show that in U2OS/GR cells, which are growth-arrested by GCs, the glucocorticoid receptor (GR) represses cyclin D1 via Tcf-beta-catenin, the transcriptional effector of the canonical Wnt pathway. We demonstrate that GR can bind beta-catenin in vitro, suggesting that GC and Wnt signaling pathways are linked directly through their effectors. Down-regulation of beta-catenin by RNA interference impeded the expression of cyclin D1 but not of c-myc or c-jun and had no significant effect on the proliferation of U2OS/GR cells. Although these results revealed that beta-catenin and cyclin D1 are not essential for the reg...Continue Reading

References

Aug 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·G H FrostE A Thompson
Oct 6, 1997·The Journal of Biological Chemistry·K OrfordS W Byers
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Apr 29, 1998·Annual Review of Physiology·K L King, J A Cidlowski
May 15, 1998·Nature·J A Lefstin, K R Yamamoto
Jun 3, 1998·The International Journal of Biochemistry & Cell Biology·M P de MoelJ J De Pont
Sep 4, 1998·Science·T C HeK W Kinzler
Nov 10, 1998·Genes & Development·B D DarimontK R Yamamoto
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·B MannC Hanski
Jun 28, 2001·Physiological Reviews·A Aranda, A Pascual
Oct 5, 2001·Nature Reviews. Molecular Cell Biology·P Cohen, S Frame
Nov 20, 2001·Nature Structural Biology·F PoyM J Eck
Jan 17, 2002·The Journal of Biological Chemistry·Fajun YangZijie Sun
Feb 22, 2002·The Journal of Biological Chemistry·David J MulhollandColleen C Nelson
Mar 6, 2002·The Journal of Biological Chemistry·Elisheva SmithBaruch Frenkel
Dec 6, 2002·Oncogene·Dennis R Chesire, William B Isaacs
Apr 18, 2003·Endocrinology and Metabolism Clinics of North America·Kenneth G Saag
May 31, 2003·Nature Cell Biology
Jun 12, 2003·The Journal of Biological Chemistry·Avital L AmirSteven P Balk
Sep 3, 2003·Biochemical and Biophysical Research Communications·Hiroko NatsumeHirotoshi Nakamura
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inez RogatskyKeith R Yamamoto
Mar 6, 2004·Science·W James Nelson, Roel Nusse
Aug 12, 2004·The Journal of Biological Chemistry·Alexander P KouzmenkoShigeaki Kato
Sep 8, 2004·Journal of Cellular Biochemistry·Boris J Cheskis
Oct 12, 2004·Gene·Jennifer J WestendorfTania M Schroeder
Feb 22, 2005·Biochemical and Biophysical Research Communications·Keizo OhnakaRyoichi Takayanagi
Aug 30, 2005·Endocrine Reviews·David J MulhollandColleen C Nelson
Jan 1, 1998·Journal of Cellular Biochemistry·Thomas J Barrett, Thomas C Spelsberg

❮ Previous
Next ❯

Citations

Nov 12, 2009·The Journal of Biological Chemistry·Sasa VukelicMarjana Tomic-Canic
Aug 22, 2007·Molecular and Cellular Biology·Hirochika KitagawaLeonard P Freedman
May 12, 2007·The Journal of Clinical Investigation·Emira AyroldiCarlo Riccardi
May 24, 2011·The Journal of Endocrinology·George SchlossmacherAnne White
May 15, 2012·Trends in Molecular Medicine·Paraskevi MoutsatsouAthanasios G Papavassiliou
Mar 13, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Jian Lu
Feb 23, 2007·The Journal of Investigative Dermatology·Lina WilliamsonEliane J Müller
Jun 26, 2007·Molecular and Cellular Endocrinology·Anna Kleiman, Jan P Tuckermann
Jan 2, 2014·Biochemical and Biophysical Research Communications·Justin K Tomblin, Travis B Salisbury
Jun 28, 2011·The American Journal of Pathology·Heather M BrechbuhlSusan D Reynolds
Nov 9, 2007·Journal of Biomolecular Screening·Michele AglerTaosheng Chen
Jul 31, 2007·Molecular and Cellular Biochemistry·Dong YuanDayong Gu
Mar 12, 2021·The American Journal of Pathology·Chenna Kesavulu SugaliWeiming Mao
May 19, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John GarciaAnne M Delany
Mar 13, 2016·The American Journal of Pathology·Salvatore PanzaStefania Catalano

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.