PMID: 6989402Mar 28, 1980Paper

The glutaminyl-transfer RNA synthetase of Escherichia coli. Purification, structure and function relationship

Biochimica Et Biophysica Acta
D KernY Boulanger

Abstract

Glutaminyl-tRNA synthetase from Escherichia coli has been purified to homogeneity with a yield of about 50%. It is a monomer of about 69 000 daltons. Arginyl and glutamyl-tRNA synthetases are also monomeric synthetases of molecular weight significantly lower than 100 000. In addition it is well known that these three synthetases require their cognate tRNA to catalyze the [32P]PPi-ATP exchange. Like arginyl-tRNA synthetase, but unlike glutamyl-tRNA synthetase, glutaminyl-tRNA synthetase seems to contain some repeated sequences. Therefore no correlation can be established between the tRNA requirement of these synthetases for the catalysis of the isotope-exchange and the presence or the absence of sequence duplication. In the native enzyme four sulfhydryl groups react with dithiobisnitrobenzoic acid causing a loss of both the aminoacylation and the [32P]PPi-ATP exchange activities. The rate-limiting steps of the overall aminoacylation and its reverse reaction correspond, respectively, to the catalysis of the aminoacylation of tRNA Gln and of the the deacylation of glutaminyl-tRNA Gln. At acidic pH, glutaminyl-tRNA synthetase catalyzes the synthesis of the glutaminyl-tRNA Gln and its deacylation at significantly lower rates than th...Continue Reading

References

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Citations

Apr 15, 2003·Journal of Molecular Biology·Timothy L BullockJohn J Perona
May 10, 2003·Structure·Luke D Sherlin, John J Perona
Jul 14, 2010·Biochemistry·Annia Rodríguez-HernándezJohn J Perona
Mar 15, 2011·Structure·Annia Rodríguez-Hernández, John J Perona
Oct 3, 1983·European Journal of Biochemistry·J C ThomesR Julien
Oct 3, 1983·European Journal of Biochemistry·M H RatinaudR Julien
Oct 6, 1980·FEBS Letters·A Joachimiak, J Barciszewski
Aug 26, 2014·Molecular Systems Biology·Matthew ScottTerence Hwa

Related Concepts

Glutamine-tRNA ligase
Amino Acyl-tRNA Synthetases
Alkalescens-Dispar Group
D-Glutamine
Glutamate-tRNA Ligase
Spermidine
Sulfhydryl Compounds

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