Abstract
Growth-blocking peptide (GBP) is a 25-amino acid cytokine isolated from the lepidopteran insect Pseudaletia separata. GBP exhibits various biological activities such as regulation of larval growth of insects, proliferation of a few kinds of cultured cells, and stimulation of a class of insect immune cells called plasmatocytes. The tertiary structure of GBP consists of a well structured core domain and disordered N and C termini. Our previous studies revealed that, in addition to the structured core, specific residues in the unstructured N-terminal region (Glu1 and Phe3) are also essential for the plasmatocyte-stimulating activity. In this study, a number of deletion, insertion, and site-directed mutants targeting the unstructured N-terminal residues of GBP were constructed to gain more detailed insight into the mode of interaction between the N-terminal region and GBP receptor. Alteration of the backbone length of the linker region between the core structure and N-terminal domain reduced plasmatocyte-stimulating activity. The substitutions of Gly5 or Gly6 in this linker region with more bulky residues, such as Phe and Pro, also remarkably reduced this activity. We conclude that the interaction of GBP with its receptor depends o...Continue Reading
References
Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Jan 1, 1981·Advances in Protein Chemistry·J S Richardson
May 17, 1994·Biochemistry·N A FarrowL E Kay
Jan 1, 1994·Journal of Biochemistry·Y Hayakawa
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 12, 1997·The Journal of Biological Chemistry·K D ClarkM R Strand
Oct 1, 1998·Biochemical and Biophysical Research Communications·Y Hayakawa, A Ohnishi
Jan 16, 1999·The Journal of Biological Chemistry·T AizawaK Nitta
Mar 5, 1999·Journal of Molecular Biology·S MineT Imoto
Mar 31, 1999·Peptides·K FuruyaD A Schooley
Aug 28, 1999·Peptides·S D HaH Kataoka
Apr 1, 2000·Journal of Insect Physiology·M R StrandK D Clark
Mar 30, 2001·The Journal of Biological Chemistry·K D ClarkM R Strand
Jun 29, 2001·The Journal of Biological Chemistry·T AizawaK Kawano
Jul 4, 2001·The Journal of Biological Chemistry·K D ClarkM R Strand
Jul 14, 2001·The Journal of Biological Chemistry·N Ohnishi, Y Takahashi
Aug 10, 2001·The Journal of Biological Chemistry·A OhnishiY Hayakawa
Dec 31, 2002·The Journal of Biological Chemistry·Masahito TadaKeiichi Kawano
Citations
Jun 5, 2013·Developmental and Comparative Immunology·Hu WanByung Rae Jin
Aug 28, 2009·The Journal of Biological Chemistry·Yoshitaka UmetsuKeiichi Kawano
May 17, 2005·Journal of Insect Physiology·Kevin D ClarkMichael R Strand