The Glycolytic Enzyme PFKFB3 Controls TNF-α-Induced Endothelial Proinflammatory Responses

Inflammation
Ruyuan ZhangYaoqing Tang

Abstract

Endothelial cells play an important role in health and a variety of diseases. Recent evidences show that endothelial cells rely on glycolysis rather than on oxidative phosphorylation to generate energy to support cellular functions such as angiogenesis. However, the effect of endothelial glycolysis on vascular inflammation remains little known. Here, we investigate the role of key glycolytic enzyme PFKFB3 in tumor necrosis factor-α (TNF-α)-induced endothelial proinflammatory responses. siRNAs were used to knockdown the expression of PFKFB3. In some experiments, PFKFB3 inhibitors were also used. TNF-α at 20 ng/ml was added to confluent endothelial cells for different time period of stimulation. PFKFB3 expression was examined by RT-PCR and western blotting. Cytokine antibody panel membranes were employed to detect different cytokines/chemokines in culture supernatant of endothelial cells. The determination of monocyte adhesion to endothelial cells after TNF-α treatment was conducted using THP-1 cells. The monocyte attraction was performed using Transwell filters. For further mechanisms, NF-κB-p65 localization was examined by immunofluorescence. Expression of total IκB, phospho-IκB, phospho-NF-κB-p65, and Ikkβ was detected by west...Continue Reading

References

Apr 5, 1983·Biochimica Et Biophysica Acta·A Dobrina, F Rossi
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·I AlkalayY Ben-Neriah
Jul 1, 1997·The American Journal of Physiology·O CulicJ Schrader
Jan 14, 1999·The New England Journal of Medicine·R Ross
Mar 6, 2002·Circulation·Peter LibbyAttilio Maseri
May 1, 2004·Critical Care Medicine·William C Aird
Nov 2, 2004·Nature Reviews. Cancer·Robert A Gatenby, Robert J Gillies
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hidenori BandoTakao Koike
Sep 26, 2007·Nature Reviews. Immunology·Jordan S Pober, William C Sessa
May 29, 2009·The Journal of Biological Chemistry·Abdullah YalcinJason Chesney
Nov 17, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Kirsten PetersC James Kirkpatrick
Aug 3, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Glenda Parra-BonillaTroy Stevens
Oct 12, 2011·Annual Review of Cell and Developmental Biology·Sophia Y Lunt, Matthew G Vander Heiden
Aug 6, 2013·Cell·Katrien De BockPeter Carmeliet
Mar 19, 2014·Nature Communications·Takehiro YamamotoMakoto Suematsu
Apr 5, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Yiming XuYuqing Huo
Feb 20, 2016·Circulation Research·Michael A Gimbrone, Guillermo García-Cardeña
Jan 14, 2017·Cell·Qian ZhangDavid Baltimore

❮ Previous
Next ❯

Citations

Apr 8, 2020·The FEBS Journal·Gonzalo Soto-HerederoMaría Mittelbrunn
Apr 5, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anahita AbdaliStefano Bellosta
Apr 27, 2021·Antioxidants & Redox Signaling·Masuko Ushio-FukaiTohru Fukai
Apr 24, 2021·Circulation Research·Wusheng XiaoJoseph Loscalzo
Jun 24, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Reece P StevensJi Young Lee
Aug 8, 2021·International Journal of Molecular Sciences·Michael G AppiahMotomu Shimaoka
May 22, 2020·ACS Medicinal Chemistry Letters·Carlo De DominicisMatteo Zanda
Oct 1, 2021·Scientific Reports·Kathrin KoedderitzschNadine Biesemann

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
Profiler
transfection
antibody array
nuclear translocation

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.