The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments

Biochemical and Biophysical Research Communications
S OgawaK Inoue


Periodic acid-Schiff-positive colloids occur in the pituitary glands of many vertebrates. These colloids are surrounded by folliculo-stellate (FS) cells and increase dramatically in number and size with age. The characterization of these colloidal substances is necessary for the establishment of FS cell function. In a previous study we showed that these colloids in the senescent pituitary contain a number of glycoproteins; however, the characterization of these glycoproteins has not yet been carried out. To characterize the pituitary colloid, we purified the colloids by centrifugation and Percoll gradient. The isolated colloids were unable to be solubilized in a regular buffer since they were very dense. However, solubilization was accomplished in sodium dodecyl sulfate (SDS) lysis buffer following treatment with exoglycosidase. Analysis using SDS-polyacrylamide gel electrophoresis showed that the porcine pituitary colloids contain four proteins, CP26, CP40, CP48 and CP60, whose estimated molecular weights were 26 kD, 36-43 kD, 48 kD, and 60 kD, respectively. Staining with concanavalin A (ConA) showed that both CP26 and CP40 were glycoproteins. Moreover, N-terminal amino acid sequencing and Western blot analysis revealed CP26, ...Continue Reading


Jan 1, 1992·Acta Neuropathologica·N H Choi-MiuraM Tomita
May 8, 1992·Brain Research·P L McGeerD G Walker
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N FerraraD Gospodarowicz
Aug 1, 1989·Molecular and Cellular Biology·R ButtyanI S Sawczuk
Jan 1, 1989·International Immunology·B F MurphyA J d'Apice
Nov 30, 1989·Biochemical and Biophysical Research Communications·D Gospodarowicz, K Lau
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·K P Dingemans, C A Feltkamp
Sep 21, 1995·Biochimica Et Biophysica Acta·R FlachC Koch-Brandt
Dec 1, 1996·Archives of Histology and Cytology·S OgawaK Inoue
Mar 1, 1953·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J F RINEHART, M G FARQUHAR
Aug 1, 1951·The Journal of Endocrinology·E G BASSETT

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