The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments

Biochemical and Biophysical Research Communications
S OgawaK Inoue

Abstract

Periodic acid-Schiff-positive colloids occur in the pituitary glands of many vertebrates. These colloids are surrounded by folliculo-stellate (FS) cells and increase dramatically in number and size with age. The characterization of these colloidal substances is necessary for the establishment of FS cell function. In a previous study we showed that these colloids in the senescent pituitary contain a number of glycoproteins; however, the characterization of these glycoproteins has not yet been carried out. To characterize the pituitary colloid, we purified the colloids by centrifugation and Percoll gradient. The isolated colloids were unable to be solubilized in a regular buffer since they were very dense. However, solubilization was accomplished in sodium dodecyl sulfate (SDS) lysis buffer following treatment with exoglycosidase. Analysis using SDS-polyacrylamide gel electrophoresis showed that the porcine pituitary colloids contain four proteins, CP26, CP40, CP48 and CP60, whose estimated molecular weights were 26 kD, 36-43 kD, 48 kD, and 60 kD, respectively. Staining with concanavalin A (ConA) showed that both CP26 and CP40 were glycoproteins. Moreover, N-terminal amino acid sequencing and Western blot analysis revealed CP26, ...Continue Reading

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Citations

Nov 28, 2001·British Journal of Haematology·A TrifunovićZ Popović
Sep 25, 1999·Archives of Histology and Cytology·K InoueS Ogawa
May 1, 2004·Zoological Science·Yoko MasekiTakafumi Sakai
Nov 23, 2006·International Journal of Legal Medicine·Takaki IshikawaHitoshi Maeda
May 21, 2005·Domestic Animal Endocrinology·C G ScanesL L Anderson
Oct 27, 2012·Cell and Tissue Research·Kotaro HoriguchiTakashi Yashiro
Jan 14, 2016·International Journal of Molecular Sciences·Saishu YoshidaYukio Kato
Jul 8, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Giacomo AzzaliGiuseppina Romita
Jul 8, 2008·Journal of Neuroendocrinology·S Devnath, K Inoue
Feb 3, 2012·Physiological Reviews·Carolina Perez-CastroEduardo Arzt

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