The glycosylation of myeloperoxidase

Biochimica Et Biophysica Acta
Tina RavnsborgPeter Højrup

Abstract

The enzyme myeloperoxidase (MPO) is an important part of the neutrophil immune reaction and can be found in alfa granula. The presence of MPO can be used to distinguish acute myelogenous leukemia from acute lymphocytic leukemia. However, the methods employed to do so, such as flow cytometry and immunohistochemistry rely on antibody recognition, and therefore the characterization of the mature MPO, including post-translational modifications, must be considered as important as epitope mapping. MPO has 5 N-linked glycosylation sites, occupied by both high mannose and complex glycan structures. In this study we utilize intact glycopeptide MSMS analysis for site specific characterization of the glycan structures of MPO from a cancer patient. The identified glycan structures are compared to those of MPO from healthy donors, in order to probe for any potential differences that may have diagnostic use.

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Citations

Jul 25, 2012·Antioxidants & Redox Signaling·Claudia NussbaumMarkus Sperandio
Jan 9, 2016·Analytical Biochemistry·Michael CaragataChamindie Punyadeera
Apr 18, 2016·Molecular Aspects of Medicine·Ian LokeMorten Thaysen-Andersen
Dec 6, 2011·Journal of Proteomics·Morten ZoegaChristian Schou
Aug 15, 2019·Biological Reviews of the Cambridge Philosophical Society·Harry C TjondroMorten Thaysen-Andersen
Nov 14, 2019·The Journal of Biological Chemistry·Karli R ReidingAlbert J R Heck
Feb 4, 2015·The Journal of Biological Chemistry·Morten Thaysen-AndersenNicolle H Packer
Mar 10, 2020·Glycobiology·Hannes HinneburgMorten Thaysen-Andersen
Dec 5, 2020·The Journal of Biological Chemistry·Harry C TjondroMorten Thaysen-Andersen
Aug 28, 2020·Molecular Aspects of Medicine·Julian UgonottiMorten Thaysen-Andersen
Feb 12, 2017·Kidney International·Jun-Tao YuMing-Hui Zhao

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