The glyoxylate shunt is essential for CO2-requiring oligotrophic growth of Rhodococcus erythropolis N9T-4

Applied Microbiology and Biotechnology
Takanori YanoHiroshi Takagi

Abstract

Rhodococcus erythropolis N9T-4 shows extremely oligotrophic growth requiring atmospheric CO2 and forms its colonies on an inorganic basal medium (BM) without any additional carbon source. Screening of a random mutation library constructed by a unique genome deletion method that we established indicated that the aceA, aceB, and pckG genes encoding isocitrate lyase, malate synthase, and phosphoenolpyruvate carboxykinase, respectively, were requisite for survival on BM plates. The aceA- and aceB deletion mutants and the pckG deletion mutant grew well on BM plates containing L-malate and D-glucose, respectively, suggesting that the glyoxylate (GO) shunt and gluconeogenesis are essential for the oligotrophic growth of N9T-4. Interestingly, most of the enzyme activities in the TCA cycle were observed in the cell-free extract of N9T-4, with perhaps the most important exception being α-ketoglutarate dehydrogenase (KGDH) activity. Instead of the KGDH activity, we detected a remarkable level of α-ketoglutarate decarboxylase (KGD) activity, which is the activity exhibited by the E1 component of the KGDH complex in Mycobacterium tuberculosis. The recombinant KGD of N9T-4 catalyzed the decarboxylation of α-ketoglutarate to form succinic sem...Continue Reading

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Citations

Nov 6, 2015·Bioscience, Biotechnology, and Biochemistry·Takanori YanoNobuyuki Yoshida
Oct 9, 2016·Applied Microbiology and Biotechnology·Nobuyuki YoshidaHiroshi Takagi
Jun 5, 2018·Bioscience, Biotechnology, and Biochemistry·Tomohiro Matsuoka, Nobuyuki Yoshida
Apr 7, 2019·Applied Microbiology and Biotechnology·Tomohiro Matsuoka, Nobuyuki Yoshida
Apr 7, 2021·Biotechnology Advances·Youxiang Liang, Huimin Yu

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