Abstract
The pancreatic islet monosialo-ganglioside (GM2-1), an autoantigen in insulin-dependent diabetes mellitus (IDDM) recently shown to be the target of autoantibodies associated with diabetes development in relatives of IDDM patients, is islet specific within the pancreas, and its expression is metabolically regulatable. In the present study we sought to establish 1) whether GM2-1 is beta-cell specific, and 2) its intracellular localization. To this end, we analyzed the pattern of ganglioside expression in highly purified beta- and non-beta-cells isolated from rat islets. In addition, ganglioside levels were determined in subcellular fractions of a rat beta-cell line (INS). No qualitative or quantitative difference was found in the pattern of ganglioside expression between beta and non-beta rat islet cells, with GM3, GM2-1, and GD3 gangliosides expressed in both cell populations. Within INS cells, GM2-1 ganglioside was expressed in the fraction containing secretory granules and, to a lesser extent, in plasma membranes; GM3 was expressed in secretory granules, whereas GD3 was found only in plasma membranes. These data indicate that the GM2-1 autoantigen is not beta-cell specific within the islets, in accordance with the observation ...Continue Reading
References
Jan 1, 1992·Endocrinology·M AsfariC B Wollheim
Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Apr 1, 1992·Diabetologia·S GenoveseG F Bottazzo
Mar 1, 1992·Diabetes·R GiananiG S Eisenbarth
Jan 1, 1992·Endocrinology·F DottaU Di Mario
Dec 1, 1991·The Journal of Clinical Endocrinology and Metabolism·L CastañoG S Eisenbarth
Jan 1, 1990·Annual Review of Immunology·L Castaño, G S Eisenbarth
Jun 14, 1990·Nature·B O RoepJ C Hutton
Dec 1, 1990·Experimental Cell Research·D G RouillerP A Halban
Oct 1, 1989·Acta Endocrinologica·S Ullrich, C B Wollheim
Nov 1, 1989·Diabetes·F DottaR C Nayak
Oct 24, 1986·Science·G Griffiths, K Simons
May 1, 1988·Diabetes·P G ColmanG S Eisenbarth
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L OrciA Perrelet
Oct 1, 1965·The Journal of Clinical Endocrinology and Metabolism·V HerbertS J Bleicher
Dec 23, 1983·Science·J P PalmerT L Paquette
Jan 1, 1981·Annual Review of Biochemistry·S Hakomori
Jan 18, 1980·Biochimica Et Biophysica Acta·L Svennerholm, P Fredman
Jul 1, 1980·Journal of Neurochemistry·M A Williams, R H McCluer
Apr 1, 1994·The Biochemical Journal·P A Halban, J C Irminger
Oct 1, 1993·The Journal of Clinical Investigation·M A Atkinson, N K Maclaren
Jan 1, 1993·The Journal of Clinical Investigation·M A AtkinsonN K Maclaren
Feb 1, 1993·Clinical Immunology and Immunopathology·F DottaG S Eisenbarth
Sep 1, 1995·Diabetologia·F DottaU Di Mario
Jun 15, 1996·The Journal of Clinical Investigation·H J AanstootS Baekkeskov
Sep 1, 1996·Diabetes·C J HawkesJ C Hutton
Sep 1, 1996·Diabetes·F DottaU Di Mario
Sep 1, 1963·Journal of Neurochemistry·L SVENNERHOLM
Jul 1, 1991·Trends in Cell Biology·G Seethaler, W Huttner
Citations
Oct 12, 2000·Molecular Biology of the Cell·M PilonH M Dosch
May 17, 2005·Diabetes/metabolism Research and Reviews·Bernd FrankeTerry J Wilkin
Sep 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jenny VikmanLena Eliasson
May 29, 2002·Cell Death and Differentiation·D MelchiorriL Lenti
Apr 8, 2019·Diabetologia·Boone M PrenticeRichard M Caprioli
Aug 6, 2020·Genes and Immunity·Katharina Kappler, Thierry Hennet
Aug 5, 1999·Autoimmunity·E AnastasiU Di Mario
Apr 22, 2021·The Biochemical Journal·J Sebastian TemmeJeffrey C Gildersleeve