The GM2-1 ganglioside islet autoantigen in insulin-dependent diabetes mellitus is expressed in secretory granules and is not beta-cell specific

Endocrinology
F DottaP A Halban

Abstract

The pancreatic islet monosialo-ganglioside (GM2-1), an autoantigen in insulin-dependent diabetes mellitus (IDDM) recently shown to be the target of autoantibodies associated with diabetes development in relatives of IDDM patients, is islet specific within the pancreas, and its expression is metabolically regulatable. In the present study we sought to establish 1) whether GM2-1 is beta-cell specific, and 2) its intracellular localization. To this end, we analyzed the pattern of ganglioside expression in highly purified beta- and non-beta-cells isolated from rat islets. In addition, ganglioside levels were determined in subcellular fractions of a rat beta-cell line (INS). No qualitative or quantitative difference was found in the pattern of ganglioside expression between beta and non-beta rat islet cells, with GM3, GM2-1, and GD3 gangliosides expressed in both cell populations. Within INS cells, GM2-1 ganglioside was expressed in the fraction containing secretory granules and, to a lesser extent, in plasma membranes; GM3 was expressed in secretory granules, whereas GD3 was found only in plasma membranes. These data indicate that the GM2-1 autoantigen is not beta-cell specific within the islets, in accordance with the observation ...Continue Reading

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Citations

May 17, 2005·Diabetes/metabolism Research and Reviews·Bernd FrankeTerry J Wilkin
Sep 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jenny VikmanLena Eliasson
Apr 8, 2019·Diabetologia·Boone M PrenticeRichard M Caprioli
Aug 6, 2020·Genes and Immunity·Katharina Kappler, Thierry Hennet
Apr 22, 2021·The Biochemical Journal·J Sebastian TemmeJeffrey C Gildersleeve

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