The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons

Neural Plasticity
Valerie T RamírezNibaldo C Inestrosa

Abstract

Mastoparan-7 (Mas-7), an analogue of the peptide mastoparan, which is derived from wasp venom, is a direct activator of Pertussis toxin- (PTX-) sensitive G proteins. Mas-7 produces several biological effects in different cell types; however, little is known about how Mas-7 influences mature hippocampal neurons. We examined the specific role of Mas-7 in the development of dendritic spines, the sites of excitatory synaptic contact that are crucial for synaptic plasticity. We report here that exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gα(o) signaling, increasing the intracellular Ca(2+) concentration. To define the role of signaling intermediates, we measured the levels of phosphorylated protein kinase C (PKC), c-Jun N-terminal kinase (JNK), and calcium-calmodulin dependent protein kinase IIα (CaMKIIα) after Mas-7 treatment and determined that CaMKII activation is necessary for the Mas-7-dependent increase in dendritic spine density. Our results demonstrate a critical role for Gα(o) subunit signaling in the regulation of synapse for...Continue Reading

References

Jul 31, 1990·Biochemical and Biophysical Research Communications·Y OzakiS Kume
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F WorleyS H Snyder
Nov 30, 1994·Biochemical and Biophysical Research Communications·B K Drøbak, P A Watkins
Dec 20, 1996·The Journal of Biological Chemistry·B C SuhK T Kim
Feb 4, 1998·Archives of Biochemistry and Biophysics·M ZorkoU Langel
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M JiangL Birnbaumer
May 18, 1999·The Journal of Biological Chemistry·C L LonglandF Michelangeli
Jun 26, 2003·The European Journal of Neuroscience·Miri Goldin, Menahem Segal
Jun 11, 2004·Nature·Masanori MatsuzakiHaruo Kasai
Jun 15, 2004·Experimental Cell Research·Alejandra R AlvarezNibaldo C Inestrosa
Jan 13, 2006·British Journal of Pharmacology·Graeme Milligan, Evi Kostenis
Jul 18, 2006·Nature Neuroscience·Jacob FerrisGregg Roman
Feb 7, 2007·Annual Review of Neuroscience·Veronica A Alvarez, Bernardo L Sabatini
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ingrid EhrlichRoberto Malinow
Feb 21, 2008·Annual Review of Neuroscience·Jennifer N Bourne, Kristen M Harris
Apr 2, 2008·The Journal of Cell Biology·Jacqueline FernandesMiguel A Valverde
Mar 31, 2009·Trends in Cell Biology·Thomas M Newpher, Michael D Ehlers
Apr 1, 2009·The Journal of Biological Chemistry·Ginny G FaríasNibaldo C Inestrosa
Nov 4, 2009·Neural Development·Lorena Varela-NallarNibaldo C Inestrosa
Jan 21, 2012·Neuro-degenerative Diseases·Lorena Varela-NallarNibaldo C Inestrosa
Feb 16, 2012·Nature Reviews. Neuroscience·John LismanSridhar Raghavachari
Jul 31, 2013·Peptides·Christopher J SampleElizabeth A Ramsburg
Apr 18, 2014·Nature Reviews. Neuroscience·Eleanor T Coffey
Aug 28, 2014·Trends in Neurosciences·Brady K AtwoodBrian N Mathur
Oct 31, 2014·Cell Death & Disease·S Di AngelantonioD Ragozzino
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Matthias HillenbrandAndreas Plückthun

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
GTPase

Software Mentioned

Imaris
NIH ImageJ
Mas
GraphPad Prism5

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

© 2022 Meta ULC. All rights reserved