The GOAT-ghrelin system is not essential for hypoglycemia prevention during prolonged calorie restriction.

PloS One
Chun-Xia YiP T Pfluger

Abstract

Ghrelin acylation by ghrelin O-acyltransferase (GOAT) has recently been reported to be essential for the prevention of hypoglycemia during prolonged negative energy balance. Using a unique set of four different genetic loss-of-function models for the GOAT/ghrelin/growth hormone secretagogue receptor (GHSR) system, we thoroughly tested the hypothesis that lack-of-ghrelin activation or signaling would lead to hypoglycemia during caloric deprivation. Male and female knockout (KO) mice for GOAT, ghrelin, GHSR, or both ghrelin and GHSR (dKO) were subjected to prolonged calorie restriction (40% of ad libitum chow intake). Body weight, fat mass, and glucose levels were recorded daily and compared to wildtype (WT) controls. Forty-eight hour blood glucose profiles were generated for each individual mouse when 2% or less body fat mass was reached. Blood samples were obtained for analysis of circulating levels of acyl- and desacyl-ghrelin, IGF-1, and insulin. Chronic calorie restriction progressively decreased body weight and body fat mass in all mice regardless of genotype. When fat mass was depleted to 2% or less of body weight for 2 consecutive days, random hypoglycemic events occurred in some mice across all genotypes. There was no in...Continue Reading

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Citations

Dec 24, 2013·The Journal of Endocrinology·Pouneh K Fazeli, Anne Klibanski
Jan 2, 2014·Scientifica·Christine Delporte
Oct 9, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Geetali PradhanYuxiang Sun
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Jan 1, 2016·Biologie aujourd'hui·Alexandra Labarthe, Virginie Tolle
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Jan 19, 2021·The FEBS Journal·María P CornejoMario Perello
Jul 24, 2021·Diabetology & Metabolic Syndrome·Behiye ÖzcanAart Jan van der Lely

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Methods Mentioned

BETA
transgenic
genetic modification
NMR

Software Mentioned

VelociGene
GraphPad Prism

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