The Golgi-localized Arabidopsis endomembrane protein12 contains both endoplasmic reticulum export and Golgi retention signals at its C terminus.

The Plant Cell
Caiji GaoLiwen Jiang

Abstract

Endomembrane proteins (EMPs), belonging to the evolutionarily conserved transmembrane nine superfamily in yeast and mammalian cells, are characterized by the presence of a large lumenal N terminus, nine transmembrane domains, and a short cytoplasmic tail. The Arabidopsis thaliana genome contains 12 EMP members (EMP1 to EMP12), but little is known about their protein subcellular localization and function. Here, we studied the subcellular localization and targeting mechanism of EMP12 in Arabidopsis and demonstrated that (1) both endogenous EMP12 (detected by EMP12 antibodies) and green fluorescent protein (GFP)-EMP12 fusion localized to the Golgi apparatus in transgenic Arabidopsis plants; (2) GFP fusion at the C terminus of EMP12 caused mislocalization of EMP12-GFP to reach post-Golgi compartments and vacuoles for degradation in Arabidopsis cells; (3) the EMP12 cytoplasmic tail contained dual sorting signals (i.e., an endoplasmic reticulum export motif and a Golgi retention signal that interacted with COPII and COPI subunits, respectively); and (4) the Golgi retention motif of EMP12 retained several post-Golgi membrane proteins within the Golgi apparatus in gain-of-function analysis. These sorting signals are highly conserved in...Continue Reading

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Citations

Jan 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Caiji GaoLiwen Jiang
Oct 6, 2015·Molecules and Cells·Hee-Kyung AhnHyun-Sook Pai
Oct 9, 2014·The Plant Journal : for Cell and Molecular Biology·Jinbo ShenLiwen Jiang
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Feb 7, 2013·The Plant Journal : for Cell and Molecular Biology·Sha LiYan Zhang
Aug 3, 2013·The Plant Journal : for Cell and Molecular Biology·Hao WangLiwen Jiang
Jun 7, 2013·The Plant Journal : for Cell and Molecular Biology·Jinbo ShenLiwen Jiang
Feb 3, 2016·Molecular Plant·Yong CuiLiwen Jiang
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May 25, 2013·Molecular Plant·Jinbo ShenLiwen Jiang
Jul 13, 2012·Trends in Plant Science·Yu DingLiwen Jiang
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Feb 6, 2018·The Plant Journal : for Cell and Molecular Biology·Yun-Ji ShinRichard Strasser
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Nov 27, 2019·International Journal of Molecular Sciences·Tianran ShiSakiko Okumoto
Apr 7, 2021·Plant Physiology·Stefan Wojcik, Verena Kriechbaumer

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