The golgin family exhibits a propensity to form condensates in living cells.

FEBS Letters
Pascal ZiltenerJames E Rothman

Abstract

The Golgi is surrounded by a ribosome-excluding matrix. Recently, we reported that the cis-Golgi-localized golgin GM130 can phase-separate to form dynamic, liquid-like condensates in vitro and in vivo. Here, we show that the overexpression of each of the remaining cis (golgin160, GMAP210)- and trans (golgin97, golgin245, GCC88, GCC185)-golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB-SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed condensates. These findings support the hypothesis that, in addition to their established roles as vesicle tethers, phase separation may be a common feature of the golgin family that contributes to Golgi organization.

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Citations

Aug 6, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Denisa Jamecna, Bruno Antonny
Nov 17, 2021·FEBS Letters·Lukas A Huber

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
scanning electron microscopy
GTPase
confocal microscopy
electron microscopy
size fractionation

Software Mentioned

SmartSEM
Dragonfly
Analyze Particles
ImageJ
GraphPad
graphpad prism

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